TY - JOUR
T1 - 5-aminolevulinic acid-conjugated gold nanoparticles for photodynamic therapy of cancer
AU - Khaing Oo, Maung Kyaw
AU - Yang, Xiaochuan
AU - Du, Henry
AU - Wang, Hongjun
PY - 2008
Y1 - 2008
N2 - Background: Effective delivery of photosensitizer to target tumor cells that causes minimal damage to healthy cells is an important requirement in photodynamic therapy of cancer. Aim: We aimed to use biocompatible gold nanoparticles as a vehicle to deliver 5-aminolevulinic (5-ALA) acid for selective and efficient photodynamic therapy. Results: Protoporphyrin IX accumulated preferentially in fibrosarcoma tumor cells treated with 5-ALA-conjugated nanoparticles while yielding significantly higher reactive oxygen species compared with that with 5-ALA. The elevation of reactive oxygen species resulted in 50% more cytotoxicity to tumor cells than that of 5-ALA alone. Selective destruction of tumor cells was also achieved using 5-ALA-conjugated nanoparticles in co-culture with dermal fibroblasts. Conclusions: 5-ALA-conjugated nanoparticles offer a new modality for selective and efficient destruction of tumor cells, with minimal damage to fibroblasts in the 5-ALA-photodynamic therapy.
AB - Background: Effective delivery of photosensitizer to target tumor cells that causes minimal damage to healthy cells is an important requirement in photodynamic therapy of cancer. Aim: We aimed to use biocompatible gold nanoparticles as a vehicle to deliver 5-aminolevulinic (5-ALA) acid for selective and efficient photodynamic therapy. Results: Protoporphyrin IX accumulated preferentially in fibrosarcoma tumor cells treated with 5-ALA-conjugated nanoparticles while yielding significantly higher reactive oxygen species compared with that with 5-ALA. The elevation of reactive oxygen species resulted in 50% more cytotoxicity to tumor cells than that of 5-ALA alone. Selective destruction of tumor cells was also achieved using 5-ALA-conjugated nanoparticles in co-culture with dermal fibroblasts. Conclusions: 5-ALA-conjugated nanoparticles offer a new modality for selective and efficient destruction of tumor cells, with minimal damage to fibroblasts in the 5-ALA-photodynamic therapy.
KW - 5-aminolevulinic acid
KW - Gold nanoparticles
KW - Photodyanamic therapy
KW - Protoporphyrin IX
KW - Reactive oxygen species
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U2 - 10.2217/17435889.3.6.777
DO - 10.2217/17435889.3.6.777
M3 - Article
C2 - 19025452
AN - SCOPUS:57149122882
SN - 1743-5889
VL - 3
SP - 777
EP - 786
JO - Nanomedicine
JF - Nanomedicine
IS - 6
ER -