TY - JOUR
T1 - A fluorescence spectroscopy approach for fast determination of β-cyclodextrin-guest binding constants
AU - Zhou, Xiaqing
AU - Liang, Jun F.
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Cyclodextrins are capable of forming host-guest inclusion complexes with a variety of guest molecules. However, binding constant measurements of various host-guest inclusions require complex equipment like NMR. Current methods may make binding constant measurement extremely difficult or even impossible for some guest molecules with specific or complicated structures. In this paper, we discovered a new method to determine the binding constants of complexes between β-Cyclodextrin and guest molecules using an inducible fluorophore, Alizarin Red S. The β-Cyclodextrin binding constants of various guest molecules including amino acids, anticancer drugs, antibiotics, and peptides were calculated according to the competition method. This novel florescence spectroscopic technique is reliable and reproducible, and represents an easy and accurate way for β-Cyclodextrin-guest binding constants measurement.
AB - Cyclodextrins are capable of forming host-guest inclusion complexes with a variety of guest molecules. However, binding constant measurements of various host-guest inclusions require complex equipment like NMR. Current methods may make binding constant measurement extremely difficult or even impossible for some guest molecules with specific or complicated structures. In this paper, we discovered a new method to determine the binding constants of complexes between β-Cyclodextrin and guest molecules using an inducible fluorophore, Alizarin Red S. The β-Cyclodextrin binding constants of various guest molecules including amino acids, anticancer drugs, antibiotics, and peptides were calculated according to the competition method. This novel florescence spectroscopic technique is reliable and reproducible, and represents an easy and accurate way for β-Cyclodextrin-guest binding constants measurement.
KW - Alizarin Red S
KW - Binding constant
KW - Fluorescence spectroscopy
KW - Host-guest inclusion
KW - β-Cyclodextrin
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U2 - 10.1016/j.jphotochem.2017.09.032
DO - 10.1016/j.jphotochem.2017.09.032
M3 - Article
AN - SCOPUS:85029504781
SN - 1010-6030
VL - 349
SP - 124
EP - 128
JO - Journal of Photochemistry and Photobiology A: Chemistry
JF - Journal of Photochemistry and Photobiology A: Chemistry
ER -