TY - GEN
T1 - A graphene nanosensor for detection of small molecules
AU - Wang, Cheng
AU - Kim, Jinho
AU - Zhu, Jing
AU - Pei, Renjun
AU - Liu, Guohua
AU - Hone, James
AU - Stojanovic, Milan
AU - Lin, Qiao
PY - 2014
Y1 - 2014
N2 - This paper presents an aptamer-based graphene nanosensor capable of detecting small molecules. To address difficulties in direct detection of small moelcules associated with their low electric charges, we use a competitive sensing approach as demonstrated with dehydroepiandrosterone sulfate (DHEA-S) as a target analyte, which is a small molecular steroid hormone with important applications in clinical diagnostics. A DHEA-S aptamer is captured by a complementary short DNA probe immobilized on the graphene and released upon exposure to DHEA-S in solution due to the binding between DHEA-S and the aptamer. The aptamer release is detected by measuring the change in the conductivity of graphene. Experimental results show that the time rate of aptamer release from the graphene is inversely proportional to DHEA-S concentration in solution. Thus, the nanosensor can potentially enable label-free, specific and quantitative measurement of DHEA-S and other small molecules.
AB - This paper presents an aptamer-based graphene nanosensor capable of detecting small molecules. To address difficulties in direct detection of small moelcules associated with their low electric charges, we use a competitive sensing approach as demonstrated with dehydroepiandrosterone sulfate (DHEA-S) as a target analyte, which is a small molecular steroid hormone with important applications in clinical diagnostics. A DHEA-S aptamer is captured by a complementary short DNA probe immobilized on the graphene and released upon exposure to DHEA-S in solution due to the binding between DHEA-S and the aptamer. The aptamer release is detected by measuring the change in the conductivity of graphene. Experimental results show that the time rate of aptamer release from the graphene is inversely proportional to DHEA-S concentration in solution. Thus, the nanosensor can potentially enable label-free, specific and quantitative measurement of DHEA-S and other small molecules.
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U2 - 10.1109/MEMSYS.2014.6765831
DO - 10.1109/MEMSYS.2014.6765831
M3 - Conference contribution
AN - SCOPUS:84898971645
SN - 9781479935086
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 1075
EP - 1078
BT - MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
T2 - 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Y2 - 26 January 2014 through 30 January 2014
ER -