TY - GEN
T1 - A microchip for nucleic acid isolation and enrichment
AU - Kim, J.
AU - Hilton, J. P.
AU - Yang, K. A.
AU - Pei, R.
AU - Ennis, K.
AU - Stojanovic, M.
AU - Lin, Q.
PY - 2012
Y1 - 2012
N2 - This paper presents a microchip that effectively isolates and enriches target-binding single-stranded DNA (ssDNA) in a randomized DNA mixture using solid-phase extraction (SPE) and electrophoresis. The microchip consists of isolation and enrichment microchambers that are connected by a microchannel partially filled with agarose gel. Single-stranded DNA oligomers in the randomized mixture are captured via specific binding onto human immunoglobulin E (IgE) that is immobilized on microbeads in the isolation chamber. Following elution, the oligomers are separated from impurities via electrophoretic transport through the gel-filled microchannel to the enrichment chamber. Experimental results show that this approach can enhance the sensitivity of ssDNA detection methods in dilute and complex biological samples.
AB - This paper presents a microchip that effectively isolates and enriches target-binding single-stranded DNA (ssDNA) in a randomized DNA mixture using solid-phase extraction (SPE) and electrophoresis. The microchip consists of isolation and enrichment microchambers that are connected by a microchannel partially filled with agarose gel. Single-stranded DNA oligomers in the randomized mixture are captured via specific binding onto human immunoglobulin E (IgE) that is immobilized on microbeads in the isolation chamber. Following elution, the oligomers are separated from impurities via electrophoretic transport through the gel-filled microchannel to the enrichment chamber. Experimental results show that this approach can enhance the sensitivity of ssDNA detection methods in dilute and complex biological samples.
UR - http://www.scopus.com/inward/record.url?scp=84860439978&partnerID=8YFLogxK
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U2 - 10.1109/MEMSYS.2012.6170298
DO - 10.1109/MEMSYS.2012.6170298
M3 - Conference contribution
AN - SCOPUS:84860439978
SN - 9781467303248
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 765
EP - 768
BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Y2 - 29 January 2012 through 2 February 2012
ER -