A microchip for nucleic acid isolation and enrichment

J. Kim, J. P. Hilton, K. A. Yang, R. Pei, K. Ennis, M. Stojanovic, Q. Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

This paper presents a microchip that effectively isolates and enriches target-binding single-stranded DNA (ssDNA) in a randomized DNA mixture using solid-phase extraction (SPE) and electrophoresis. The microchip consists of isolation and enrichment microchambers that are connected by a microchannel partially filled with agarose gel. Single-stranded DNA oligomers in the randomized mixture are captured via specific binding onto human immunoglobulin E (IgE) that is immobilized on microbeads in the isolation chamber. Following elution, the oligomers are separated from impurities via electrophoretic transport through the gel-filled microchannel to the enrichment chamber. Experimental results show that this approach can enhance the sensitivity of ssDNA detection methods in dilute and complex biological samples.

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages765-768
Number of pages4
DOIs
StatePublished - 2012
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 29 Jan 20122 Feb 2012

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

Conference2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Country/TerritoryFrance
CityParis
Period29/01/122/02/12

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