A microfluidic device for isolation of affinity oligonucleotides using combined electrokinetic and hydrodynamic manipulation

T. Olsen, J. Zhu, J. Kim, R. Pei, M. Stojanovic, Q. Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

This paper presents a microfluidic device for isolating and amplifying target-binding oligomers in which affinity-selected target-binding oligomers are electrokinetically transferred for amplification, while the amplification product is transferred back for affinity-selection via pressure-driven fluid flow. This hybrid oligomer transfer approach combines the advantages of the individual electrokinetic or pressure-driven flow methods to efficiently and effectively close the loop of iterative affinity-selection and amplification. Furthermore, the approach minimizes the limitations of either transfer method used independently by reducing the number of pressure-driven flow components (e.g. microvalves) and avoiding electrolysis-induced affinity-selection environmental conditions (e.g. pH) that can potentially compromise the target integrity.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Pages1521-1523
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/TerritoryUnited States
CitySan Antonio
Period26/10/1430/10/14

Keywords

  • Affinity binding
  • Aptamer
  • Electrophoresis
  • Polymerase chain reaction

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