TY - GEN
T1 - A microfluidic device for isolation of affinity oligonucleotides using combined electrokinetic and hydrodynamic manipulation
AU - Olsen, T.
AU - Zhu, J.
AU - Kim, J.
AU - Pei, R.
AU - Stojanovic, M.
AU - Lin, Q.
N1 - Publisher Copyright:
© 14CBMS.
PY - 2014
Y1 - 2014
N2 - This paper presents a microfluidic device for isolating and amplifying target-binding oligomers in which affinity-selected target-binding oligomers are electrokinetically transferred for amplification, while the amplification product is transferred back for affinity-selection via pressure-driven fluid flow. This hybrid oligomer transfer approach combines the advantages of the individual electrokinetic or pressure-driven flow methods to efficiently and effectively close the loop of iterative affinity-selection and amplification. Furthermore, the approach minimizes the limitations of either transfer method used independently by reducing the number of pressure-driven flow components (e.g. microvalves) and avoiding electrolysis-induced affinity-selection environmental conditions (e.g. pH) that can potentially compromise the target integrity.
AB - This paper presents a microfluidic device for isolating and amplifying target-binding oligomers in which affinity-selected target-binding oligomers are electrokinetically transferred for amplification, while the amplification product is transferred back for affinity-selection via pressure-driven fluid flow. This hybrid oligomer transfer approach combines the advantages of the individual electrokinetic or pressure-driven flow methods to efficiently and effectively close the loop of iterative affinity-selection and amplification. Furthermore, the approach minimizes the limitations of either transfer method used independently by reducing the number of pressure-driven flow components (e.g. microvalves) and avoiding electrolysis-induced affinity-selection environmental conditions (e.g. pH) that can potentially compromise the target integrity.
KW - Affinity binding
KW - Aptamer
KW - Electrophoresis
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=84931063655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84931063655&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84931063655
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 1521
EP - 1523
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -