TY - JOUR
T1 - A modified in-vitro method to assess bioavailable arsenic in pesticide-applied soils
AU - Sarkar, Dibyendu
AU - Datta, Rupali
PY - 2003/12/1
Y1 - 2003/12/1
N2 - An incubation study was designed to modify the existing in vitro methods to increase the accuracy in estimation of bioavailable arsenic in pesticide-applied soils. In addition to simulating arsenic dissolution in gastric and intestinal solutions, absorbtion by the intestinal membrane was also mimicked using iron-oxide coated filter paper strips inserted in nylon bags. The in vitro experiments were sequentially performed in two phases, namely, the stomach phase and the absorbed-intestinal phase. Arsenic extraction in the in vitro absorbed intestinal phase increased, therby making it more comparable to the potential in vivo arsenic pool. While animal studies are needed to verify the in vitro results, preliminary data indicate that this modified method may be able to improve site-specific bioavailability predictions in arsenic-contaminated soils.
AB - An incubation study was designed to modify the existing in vitro methods to increase the accuracy in estimation of bioavailable arsenic in pesticide-applied soils. In addition to simulating arsenic dissolution in gastric and intestinal solutions, absorbtion by the intestinal membrane was also mimicked using iron-oxide coated filter paper strips inserted in nylon bags. The in vitro experiments were sequentially performed in two phases, namely, the stomach phase and the absorbed-intestinal phase. Arsenic extraction in the in vitro absorbed intestinal phase increased, therby making it more comparable to the potential in vivo arsenic pool. While animal studies are needed to verify the in vitro results, preliminary data indicate that this modified method may be able to improve site-specific bioavailability predictions in arsenic-contaminated soils.
KW - Arsenic
KW - Bioavailability
KW - In-vitro Method
KW - Risk Assessment
KW - Soil
UR - http://www.scopus.com/inward/record.url?scp=0042831332&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042831332&partnerID=8YFLogxK
U2 - 10.1016/S0269-7491(03)00268-9
DO - 10.1016/S0269-7491(03)00268-9
M3 - Article
C2 - 12963297
AN - SCOPUS:0042831332
SN - 0269-7491
VL - 126
SP - 363
EP - 366
JO - Environmental Pollution
JF - Environmental Pollution
IS - 3
ER -