TY - JOUR
T1 - A surface-enhanced Raman scattering-based probe method for detecting chromogranin A in adrenal tumors
AU - Cui, Xiaoyu
AU - Hu, Dayu
AU - Wang, Chengyuan
AU - Chen, Shuo
AU - Zhao, Zeyin
AU - Xu, Xiaosong
AU - Yao, Yudong
AU - Liu, Tao
N1 - Publisher Copyright:
© 2020 Future Medicine Ltd.
PY - 2020
Y1 - 2020
N2 - Aim: We aim to demonstrate that a surface-enhanced Raman spectroscopy (SERS) probe can be effectively used for protein detection in adrenal tumors. Materials & methods: The SERS probe method, which uses Au@Ag core-shell nanoparticles conjugated with a CgA antibody and a SERS reporter, was applied to detect CgA in adrenal tumors. Results: Our data reveal that the results of the CgA-SERS probe method were almost identical to those of western blot and superior to those of traditional immunohistochemistry. Conclusion: This study offers a novel strategy to detect CgA in adrenal tumors and provides more reliable protein test results than traditional immunohistochemistry analysis for adrenal pathologists, meaning that it might be a better clinical reference for the diagnosis of pheochromocytoma.
AB - Aim: We aim to demonstrate that a surface-enhanced Raman spectroscopy (SERS) probe can be effectively used for protein detection in adrenal tumors. Materials & methods: The SERS probe method, which uses Au@Ag core-shell nanoparticles conjugated with a CgA antibody and a SERS reporter, was applied to detect CgA in adrenal tumors. Results: Our data reveal that the results of the CgA-SERS probe method were almost identical to those of western blot and superior to those of traditional immunohistochemistry. Conclusion: This study offers a novel strategy to detect CgA in adrenal tumors and provides more reliable protein test results than traditional immunohistochemistry analysis for adrenal pathologists, meaning that it might be a better clinical reference for the diagnosis of pheochromocytoma.
KW - Raman imaging
KW - chromogranin A
KW - immunohistochemistry
KW - pheochromocytoma
KW - surface-enhanced Raman scattering
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U2 - 10.2217/nnm-2019-0436
DO - 10.2217/nnm-2019-0436
M3 - Article
C2 - 31983270
AN - SCOPUS:85079351107
SN - 1743-5889
VL - 15
SP - 397
EP - 407
JO - Nanomedicine
JF - Nanomedicine
IS - 4
ER -