TY - JOUR
T1 - Absorption-based hyperspectral imaging and analysis of single erythrocytes
AU - Lee, Ji Youn
AU - Clarke, Matthew L.
AU - Tokumasu, Fuyuki
AU - Lesoine, John F.
AU - Allen, David W.
AU - Chang, Robert
AU - Litorja, Maritoni
AU - Hwang, Jeeseong
PY - 2012
Y1 - 2012
N2 - We report an absorption-based hyperspectral imaging and analysis technique to resolve unique physicochemical characteristics of subcellular substances in single erythrocytes. We constructed a microscope system installed with a spectral light engine capable of controlling the spectral shape of the illumination light by a digital micromirror device. The hyperspectral imaging system and the sequential maximum angle convex cone algorithm allow us to extract unique spectral signatures (i.e., endmembers) for different types of hemoglobin, such as oxyhemoglobin, methemoglobin, and hemozoin, and scatter from cell membrane in single erythrocytes. Further statistical endmember analysis, conducted on the hyperspectral image data, provides the abundances of specific endmembers, which can be used to build intracellular maps of the distribution of substances of interest. In addition, we perform modeling based on Mie theory to explain the scattering signatures as a function of scattering angle. The developed imaging and analysis technique enables label-free molecular imaging of endogenous biomarkers in single erythrocytes in order to build oxymetric standards on a cellular level and ultimately for in vivo as well.
AB - We report an absorption-based hyperspectral imaging and analysis technique to resolve unique physicochemical characteristics of subcellular substances in single erythrocytes. We constructed a microscope system installed with a spectral light engine capable of controlling the spectral shape of the illumination light by a digital micromirror device. The hyperspectral imaging system and the sequential maximum angle convex cone algorithm allow us to extract unique spectral signatures (i.e., endmembers) for different types of hemoglobin, such as oxyhemoglobin, methemoglobin, and hemozoin, and scatter from cell membrane in single erythrocytes. Further statistical endmember analysis, conducted on the hyperspectral image data, provides the abundances of specific endmembers, which can be used to build intracellular maps of the distribution of substances of interest. In addition, we perform modeling based on Mie theory to explain the scattering signatures as a function of scattering angle. The developed imaging and analysis technique enables label-free molecular imaging of endogenous biomarkers in single erythrocytes in order to build oxymetric standards on a cellular level and ultimately for in vivo as well.
KW - Absorption spectra of hemoglobin
KW - endmember analysis
KW - erythrocyte (red blood cell)
KW - hyperspectral imaging
KW - label-free molecular imaging
KW - scattering signature
KW - sequential maximum angle convex cone (SMACC) algorithm
KW - spectral light engine
UR - http://www.scopus.com/inward/record.url?scp=84862280463&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862280463&partnerID=8YFLogxK
U2 - 10.1109/JSTQE.2011.2164239
DO - 10.1109/JSTQE.2011.2164239
M3 - Article
AN - SCOPUS:84862280463
SN - 1077-260X
VL - 18
SP - 1130
EP - 1139
JO - IEEE Journal of Selected Topics in Quantum Electronics
JF - IEEE Journal of Selected Topics in Quantum Electronics
IS - 3
M1 - 5981373
ER -