TY - GEN
T1 - Acute spatiotemporal changes in neuronal density surrounding microelectrode arrays implanted in rat motor cortex
AU - McConnell, George C.
AU - Schneider, Thomas M.
AU - Bellamkonda, Ravi V.
PY - 2007
Y1 - 2007
N2 - Neuronal density and proximity to recording electrodes is perhaps the best indicator of recording longevity from chronically implanted microelectrodes. However, little is known about this important parameter in the hours following implantation. In this study, 1 hour, 24 hours, and 2 weeks post implantation with Si "Michigan" arrays, rats were sacrificed and cortical sections were obtained and immunostained with the neuron-specific marker NeuN. Images were quantified and compared between time points within a zone of interest (0-100 μm from the insertion sites) and examined as a function of depth below the cortical surface. Differences in neuronal density between these three time points were dependent on cortical depth. These data strongly suggest that neuronal density in the cortex respond differently to injury based on their location within the cortical column. The need to account for depth information in evaluating tissue reaction to microelectrode implantation is highlighted.
AB - Neuronal density and proximity to recording electrodes is perhaps the best indicator of recording longevity from chronically implanted microelectrodes. However, little is known about this important parameter in the hours following implantation. In this study, 1 hour, 24 hours, and 2 weeks post implantation with Si "Michigan" arrays, rats were sacrificed and cortical sections were obtained and immunostained with the neuron-specific marker NeuN. Images were quantified and compared between time points within a zone of interest (0-100 μm from the insertion sites) and examined as a function of depth below the cortical surface. Differences in neuronal density between these three time points were dependent on cortical depth. These data strongly suggest that neuronal density in the cortex respond differently to injury based on their location within the cortical column. The need to account for depth information in evaluating tissue reaction to microelectrode implantation is highlighted.
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U2 - 10.1109/CNE.2007.369631
DO - 10.1109/CNE.2007.369631
M3 - Conference contribution
AN - SCOPUS:34548717617
SN - 1424407923
SN - 9781424407927
T3 - Proceedings of the 3rd International IEEE EMBS Conference on Neural Engineering
SP - 137
EP - 140
BT - Proceedings of the 3rd International IEEE EMBS Conference on Neural Engineering
T2 - 3rd International IEEE EMBS Conference on Neural Engineering
Y2 - 2 May 2007 through 5 May 2007
ER -