An electrokinetically controlled microchip for studying binding interactions of nucleic acids with cells

J. Kim, J. P. Hilton, K. Yang, R. Pei, M. Stojanovic, Q. Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This paper presents an electrokinetically controlled microfluidic chip that allows investigation of binding interactions between nucleic acids and target cells. The microchip consists of two microchambers for capture of cells using an integrated microweir structure and collection of DNA strands, respectively. The chambers are connected by a microchannel filled with agarose gel. In the chip, nucleic acids that interact with target cells are electrokinetically separated and isolated from the cells through the gel-filled channel for subsequent analysis. Experimental results demonstrate that our microchip can potentially be used for studying interactions of nucleic acids with target cells in practical biomedical applications.

Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII
Subtitle of host publicationThe 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages1190-1193
Number of pages4
DOIs
StatePublished - 2013
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain
Duration: 16 Jun 201320 Jun 2013

Publication series

Name2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Conference

Conference2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/TerritorySpain
CityBarcelona
Period16/06/1320/06/13

Keywords

  • MCF-7 cells
  • Microfluidic chip
  • aptamers
  • electrophoresis
  • nucleic acids

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