Antibody-functionalized magnetic nanowires for cell purification

Ning Gao, Xiaochuan Yang, Yao Tsan Tsai, Geh Meh Chu, Hongjun Wang, Eui Hyeok Yang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

8 Scopus citations

Abstract

Rapid and efficient cell purification remains challenging. The use of ferromagnetic Ni nanowires for cell purification is considered superior over magnetic beads. In this study, we explored the opportunity to improve cell purification by using antibody-functionalized Ni nanowires. Antibody (anti-CD31) against mouse endothelial cells (MS-1) was conjugated to Ni nanowire surface by self-assembled monolayers (SAMs) and chemical covalent reaction. The antibody functionalized nanowires were used to purify the MS-1 from a mixture of MS-1 and mouse fibroblast cells (3T3). The nanowire-bound cells were magnetically separated to determine the separation yield of target cells. Furthermore, the proliferation of nanowire-bound cells was studied by MTT cell proliferation assay. This work demonstrates that antibody-functionalized Ni nanowires provide an effective mean to separate cells.

Original languageEnglish
Title of host publicationMicro- and Nanotechnology Sensors, Systems, and Applications
DOIs
StatePublished - 2009
EventMicro- and Nanotechnology Sensors, Systems, and Applications - Orlando, FL, United States
Duration: 15 Apr 200917 Apr 2009

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7318
ISSN (Print)0277-786X

Conference

ConferenceMicro- and Nanotechnology Sensors, Systems, and Applications
Country/TerritoryUnited States
CityOrlando, FL
Period15/04/0917/04/09

Keywords

  • Antibody
  • Functionalization
  • Magnetic nanowires
  • Mixed cells
  • Separate

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