TY - JOUR
T1 - Application of Argon Plasma Technology for the Synthesis of Anti-Infective Copper Nanoparticles
AU - Mafla, Camila
AU - Kolenovic, Belmin
AU - Centeno, Daniel
AU - Darwish, Janeen
AU - Cabinian, Katrina
AU - Richards, Kyle
AU - Cattabiani, Thomas
AU - Nunez, Jonathan
AU - Drwiega, Thomas J.
AU - Li, Wanlu
AU - Iwanicki, Marcin
AU - Sciorra, Leonard
AU - Li, Clive
AU - Traba, Christian
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2024/3/18
Y1 - 2024/3/18
N2 - The synthesis of copper nanoparticles (CuNPs) was accomplished by using a rapid, green, and versatile argon plasma reduction method that involves solvent extraction. With this method, a plasma-solid state interaction forms and CuNPs can be synthesized from copper(II) sulfate using a low-pressure, low-temperature argon plasma. Characterization studies of the CuNPs revealed that when a metal precursor is treated under optimal experimental conditions of 80 W of argon plasma for 300 s, brown CuNPs are synthesized. However, when those same brown CuNPs are placed in Milli-Q water for a period of 10 days, oxidation occurs and green CuNPs are formed. Confirmation of the chemical identity of the CuNPs was performed by using X-ray photoelectron spectroscopy. The results reveal that the brown CuNPs are predominantly Cu0 or what we refer to as CuNPs, while the green CuNPs are a mixture of Cu0 and Cu(OH)2 NPs. Upon further characterization of both brown and green CuNPs with scanning electron microscopy (SEM), the results depict brown CuNPs with a rod-like shape and approximate dimensions of 40 nm × 160 nm, while the green CuNPs were smaller in size, with dimensions of 40-80 nm, and more of a round shape. When testing the antibacterial activity of both brown and green CuNPs, our findings demonstrate the effectiveness of both CuNPs against Escherichia coli and Staphylococcus aureus bacteria at a concentration of 17 μg/mL. The inactivation of S. aureus and E. coli 7-day-old biofilms required CuNP concentrations of 99 μg/mL. SEM images of treated 7-day-old S. aureus and E. coli biofilms depict cell membranes that are completely damaged, suggesting a physical killing mechanism. In addition, when the same concentration of CuNPs used to inactivate biofilms were tested with human fibroblasts, both brown and green CuNPs were found to be biocompatible.
AB - The synthesis of copper nanoparticles (CuNPs) was accomplished by using a rapid, green, and versatile argon plasma reduction method that involves solvent extraction. With this method, a plasma-solid state interaction forms and CuNPs can be synthesized from copper(II) sulfate using a low-pressure, low-temperature argon plasma. Characterization studies of the CuNPs revealed that when a metal precursor is treated under optimal experimental conditions of 80 W of argon plasma for 300 s, brown CuNPs are synthesized. However, when those same brown CuNPs are placed in Milli-Q water for a period of 10 days, oxidation occurs and green CuNPs are formed. Confirmation of the chemical identity of the CuNPs was performed by using X-ray photoelectron spectroscopy. The results reveal that the brown CuNPs are predominantly Cu0 or what we refer to as CuNPs, while the green CuNPs are a mixture of Cu0 and Cu(OH)2 NPs. Upon further characterization of both brown and green CuNPs with scanning electron microscopy (SEM), the results depict brown CuNPs with a rod-like shape and approximate dimensions of 40 nm × 160 nm, while the green CuNPs were smaller in size, with dimensions of 40-80 nm, and more of a round shape. When testing the antibacterial activity of both brown and green CuNPs, our findings demonstrate the effectiveness of both CuNPs against Escherichia coli and Staphylococcus aureus bacteria at a concentration of 17 μg/mL. The inactivation of S. aureus and E. coli 7-day-old biofilms required CuNP concentrations of 99 μg/mL. SEM images of treated 7-day-old S. aureus and E. coli biofilms depict cell membranes that are completely damaged, suggesting a physical killing mechanism. In addition, when the same concentration of CuNPs used to inactivate biofilms were tested with human fibroblasts, both brown and green CuNPs were found to be biocompatible.
KW - antibacterial
KW - biocompatible
KW - copper
KW - green
KW - nanoparticles
KW - plasma reduction
UR - http://www.scopus.com/inward/record.url?scp=85186676395&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85186676395&partnerID=8YFLogxK
U2 - 10.1021/acsabm.3c01097
DO - 10.1021/acsabm.3c01097
M3 - Article
C2 - 38437727
AN - SCOPUS:85186676395
VL - 7
SP - 1588
EP - 1599
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 3
ER -