TY - JOUR
T1 - Assessing age-related changes in the biomechanical properties of rabbit lens using a coaligned ultrasound and optical coherence elastography system
AU - Wu, Chen
AU - Han, Zhaolong
AU - Wang, Shang
AU - Li, Jiasong
AU - Singh, Manmohan
AU - Liu, Chih Hao
AU - Aglyamov, Salavat
AU - Emelianov, Stanislav
AU - Manns, Fabrice
AU - Larin, Kirill V.
N1 - Publisher Copyright:
© 2015 The Association for Research in Vision and Ophthalmology, Inc.
PY - 2015
Y1 - 2015
N2 - Purpose. To evaluate the capability of a novel, coaligned focused ultrasound and phase-sensitive optical coherence elastography (US-OCE) system to assess age-related changes in biomechanical properties of the crystalline lens in situ. Methods. Low-amplitude elastic deformations in young and mature rabbit lenses were measured by an US-OCE system consisting of a spectral-domain optical coherence tomography (OCT) system coaligned with a focused ultrasound system used to produce a transient force on the lens surface. Uniaxial compressional tests were used to validate the OCE data. Results. The OCE measurements showed that the maximum displacements of the young rabbit lenses were significantly larger than those of the mature lenses, indicating a gradual increase of the lens stiffness with age. Temporal analyses of the displacements also demonstrate a similar trend of elastic properties in these lenses. The stress-strain measurements using uniaxial mechanical tests confirmed the results obtained by the US-OCE system. Conclusions. The results demonstrate that the US-OCE system can be used for noninvasive analysis and quantification of lens biomechanical properties in situ and possibly in vivo.
AB - Purpose. To evaluate the capability of a novel, coaligned focused ultrasound and phase-sensitive optical coherence elastography (US-OCE) system to assess age-related changes in biomechanical properties of the crystalline lens in situ. Methods. Low-amplitude elastic deformations in young and mature rabbit lenses were measured by an US-OCE system consisting of a spectral-domain optical coherence tomography (OCT) system coaligned with a focused ultrasound system used to produce a transient force on the lens surface. Uniaxial compressional tests were used to validate the OCE data. Results. The OCE measurements showed that the maximum displacements of the young rabbit lenses were significantly larger than those of the mature lenses, indicating a gradual increase of the lens stiffness with age. Temporal analyses of the displacements also demonstrate a similar trend of elastic properties in these lenses. The stress-strain measurements using uniaxial mechanical tests confirmed the results obtained by the US-OCE system. Conclusions. The results demonstrate that the US-OCE system can be used for noninvasive analysis and quantification of lens biomechanical properties in situ and possibly in vivo.
KW - Biomechanics
KW - Lens
KW - Optical coherence elastography
UR - http://www.scopus.com/inward/record.url?scp=84923289102&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923289102&partnerID=8YFLogxK
U2 - 10.1167/iovs.14-15654
DO - 10.1167/iovs.14-15654
M3 - Article
C2 - 25613945
AN - SCOPUS:84923289102
SN - 0146-0404
VL - 56
SP - 1292
EP - 1300
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 2
ER -