TY - JOUR
T1 - Bacterial Detection and Differentiation of Staphylococcus aureus and Escherichia coli Utilizing Long-Period Fiber Gratings Functionalized with Nanoporous Coated Structures
AU - He, Shuyue
AU - Wang, Jue
AU - Yang, Fan
AU - Chang, Tzu Lan
AU - Tang, Ziyu
AU - Liu, Kai
AU - Liu, Shuli
AU - Tian, Fei
AU - Liang, Jun Feng
AU - Du, Henry
AU - Liu, Yi
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/4
Y1 - 2023/4
N2 - A biosensor utilizing long-period fiber gratings (LPFG) functionalized with nanoporous coated structures was developed for the rapid detection of Staphylococcus aureus (S. aureus) bacteria. The nanoporous structure coatings on the LPFG surface facilitated specific adhesion and interaction with S. aureus, resulting in an instantaneous shift in the resonance wavelength (RW) in the transmission spectrum of the LPFG. The LPFG with nanoporous polyelectrolyte coatings exhibited an approximately seven-fold RW shift compared to the bare LPFG under the optimal experiment conditions. By tracking the RW shifts, we were able to monitor the real-time S. aureus adhesion to study the interaction process in detail. The bacterial differentiation and S. aureus specificity of the method was confirmed through a series of studies using Escherichia coli (E. coli). This nanoporous structure-enabled LPFG-based biosensor scheme holds significant promise for rapid, reliable, and low-cost detection of S. aureus for biomedical applications.
AB - A biosensor utilizing long-period fiber gratings (LPFG) functionalized with nanoporous coated structures was developed for the rapid detection of Staphylococcus aureus (S. aureus) bacteria. The nanoporous structure coatings on the LPFG surface facilitated specific adhesion and interaction with S. aureus, resulting in an instantaneous shift in the resonance wavelength (RW) in the transmission spectrum of the LPFG. The LPFG with nanoporous polyelectrolyte coatings exhibited an approximately seven-fold RW shift compared to the bare LPFG under the optimal experiment conditions. By tracking the RW shifts, we were able to monitor the real-time S. aureus adhesion to study the interaction process in detail. The bacterial differentiation and S. aureus specificity of the method was confirmed through a series of studies using Escherichia coli (E. coli). This nanoporous structure-enabled LPFG-based biosensor scheme holds significant promise for rapid, reliable, and low-cost detection of S. aureus for biomedical applications.
KW - Escherichia coli
KW - Staphylococcus aureus
KW - biosensor
KW - long-period fiber gratings
KW - nanoporous structure
UR - http://www.scopus.com/inward/record.url?scp=85156096256&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85156096256&partnerID=8YFLogxK
U2 - 10.3390/coatings13040778
DO - 10.3390/coatings13040778
M3 - Article
AN - SCOPUS:85156096256
VL - 13
JO - Coatings
JF - Coatings
IS - 4
M1 - 778
ER -