TY - JOUR
T1 - Biomimetic LBL structured nanofibrous matrices assembled by chitosan/collagen for promoting wound healing
AU - Huang, Rong
AU - Li, Wangzhou
AU - Lv, Xiaoxing
AU - Lei, Zhanjun
AU - Bian, Yongqian
AU - Deng, Hongbing
AU - Wang, Hongjun
AU - Li, Jinqing
AU - Li, Xueyong
N1 - Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - This paper reports the fabrication of biomimetic nanofibrous matrices via co-electrospinning of polycaprolactone (PCL)/cellulose acetate (CA) and layer-by-layer self-assembly (LBL) of positively charged chitosan (CS) and negatively charged Type [U+2160] collagen on the nanofibrous matrix. FE-SEM images indicate that the average fiber diameter increased from 392 to 541nm when the coating bilayers varied from 5 to 20.5. Besides, the excellent biocompatibility and enhanced attachment and spreading of normal human dermal fibroblasts (NHDFs) of prepared nanofibrous mats are confirmed by MTT and SEM results. Furthermore, the LBL structured (CS/collagen)n nanofibrous mats greatly improve the cell migration invitro, promote re-epithelialization and vascularization invivo, and up-regulate the expression of collagen [U+2163] and α-tubulin, as well as the Integrin β1 and phosphorylation of focal adhesion kinase (FAK) at Tyr-397. The levels of expressed protein are significantly enhanced with increasing coating bilayers via immunohistochemistry and western blotting analyses. Collectively, these results suggest that the LBL structured biomimetic nanofibrous matrices may enhance cell migration and further promote the skin regeneration by up-regulating the secretion of ECM protein and triggering Integrin/FAK signaling pathway, which demonstrate the potential use of the nanofibrous mats to rapidly restore the structural and functional properties of wounded skin.
AB - This paper reports the fabrication of biomimetic nanofibrous matrices via co-electrospinning of polycaprolactone (PCL)/cellulose acetate (CA) and layer-by-layer self-assembly (LBL) of positively charged chitosan (CS) and negatively charged Type [U+2160] collagen on the nanofibrous matrix. FE-SEM images indicate that the average fiber diameter increased from 392 to 541nm when the coating bilayers varied from 5 to 20.5. Besides, the excellent biocompatibility and enhanced attachment and spreading of normal human dermal fibroblasts (NHDFs) of prepared nanofibrous mats are confirmed by MTT and SEM results. Furthermore, the LBL structured (CS/collagen)n nanofibrous mats greatly improve the cell migration invitro, promote re-epithelialization and vascularization invivo, and up-regulate the expression of collagen [U+2163] and α-tubulin, as well as the Integrin β1 and phosphorylation of focal adhesion kinase (FAK) at Tyr-397. The levels of expressed protein are significantly enhanced with increasing coating bilayers via immunohistochemistry and western blotting analyses. Collectively, these results suggest that the LBL structured biomimetic nanofibrous matrices may enhance cell migration and further promote the skin regeneration by up-regulating the secretion of ECM protein and triggering Integrin/FAK signaling pathway, which demonstrate the potential use of the nanofibrous mats to rapidly restore the structural and functional properties of wounded skin.
KW - Chitosan
KW - Collagen
KW - Integrin β1 signaling
KW - Nanofibrous matrix
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=84927949362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84927949362&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2015.02.076
DO - 10.1016/j.biomaterials.2015.02.076
M3 - Article
C2 - 25890707
AN - SCOPUS:84927949362
SN - 0142-9612
VL - 53
SP - 58
EP - 75
JO - Biomaterials
JF - Biomaterials
ER -