TY - JOUR
T1 - Cation-crosslinked κ-carrageenan sub-microgel medium for high-quality embedded bioprinting
AU - Zhang, Hua
AU - Luo, Yang
AU - Hu, Zeming
AU - Chen, Mengxi
AU - Chen, Shang
AU - Yao, Yudong
AU - Yao, Jie
AU - Shao, Xiaoqi
AU - Wu, Kerong
AU - Zhu, Yabin
AU - Fu, Jun
N1 - Publisher Copyright:
© 2024 The Author(s). Published by IOP Publishing Ltd.
PY - 2024/4/1
Y1 - 2024/4/1
N2 - Three-dimensional (3D) bioprinting embedded within a microgel bath has emerged as a promising strategy for creating intricate biomimetic scaffolds. However, it remains a great challenge to construct tissue-scale structures with high resolution by using embedded 3D bioprinting due to the large particle size and polydispersity of the microgel medium, as well as its limited cytocompatibility. To address these issues, novel uniform sub-microgels of cell-friendly cationic-crosslinked kappa-carrageenan (κ-Car) are developed through an easy-to-operate mechanical grinding strategy. These κ-Car sub-microgels maintain a uniform submicron size of around 642 nm and display a rapid jamming-unjamming transition within 5 s, along with excellent shear-thinning and self-healing properties, which are critical for the high resolution and fidelity in the construction of tissue architecture via embedded 3D bioprinting. Utilizing this new sub-microgel medium, various intricate 3D tissue and organ structures, including the heart, lungs, trachea, branched vasculature, kidney, auricle, nose, and liver, are successfully fabricated with delicate fine structures and high shape fidelity. Moreover, the bone marrow mesenchymal stem cells encapsulated within the printed constructs exhibit remarkable viability exceeding 92.1% and robust growth. This κ-Car sub-microgel medium offers an innovative avenue for achieving high-quality embedded bioprinting, facilitating the fabrication of functional biological constructs with biomimetic structural organizations.
AB - Three-dimensional (3D) bioprinting embedded within a microgel bath has emerged as a promising strategy for creating intricate biomimetic scaffolds. However, it remains a great challenge to construct tissue-scale structures with high resolution by using embedded 3D bioprinting due to the large particle size and polydispersity of the microgel medium, as well as its limited cytocompatibility. To address these issues, novel uniform sub-microgels of cell-friendly cationic-crosslinked kappa-carrageenan (κ-Car) are developed through an easy-to-operate mechanical grinding strategy. These κ-Car sub-microgels maintain a uniform submicron size of around 642 nm and display a rapid jamming-unjamming transition within 5 s, along with excellent shear-thinning and self-healing properties, which are critical for the high resolution and fidelity in the construction of tissue architecture via embedded 3D bioprinting. Utilizing this new sub-microgel medium, various intricate 3D tissue and organ structures, including the heart, lungs, trachea, branched vasculature, kidney, auricle, nose, and liver, are successfully fabricated with delicate fine structures and high shape fidelity. Moreover, the bone marrow mesenchymal stem cells encapsulated within the printed constructs exhibit remarkable viability exceeding 92.1% and robust growth. This κ-Car sub-microgel medium offers an innovative avenue for achieving high-quality embedded bioprinting, facilitating the fabrication of functional biological constructs with biomimetic structural organizations.
KW - biomimetic constructs
KW - embedded bioprinting
KW - jammed sub-microgel bath
KW - mechanical grinding
KW - κ-carrageenan hydrogel
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UR - http://www.scopus.com/inward/citedby.url?scp=85184009077&partnerID=8YFLogxK
U2 - 10.1088/1758-5090/ad1cf3
DO - 10.1088/1758-5090/ad1cf3
M3 - Article
C2 - 38198708
AN - SCOPUS:85184009077
SN - 1758-5082
VL - 16
JO - Biofabrication
JF - Biofabrication
IS - 2
M1 - 025009
ER -