TY - JOUR
T1 - Cell growth as a sheet on three-dimensional sharp-tip nanostructures
AU - Choi, Chang Hwan
AU - Heydarkhan-Hagvall, Sepideh
AU - Wu, Benjamin M.
AU - Dunn, James C.Y.
AU - Beygui, Ramin E.
AU - Cjkim, Chang Jin
PY - 2009/6/1
Y1 - 2009/6/1
N2 - Cells in vivo encounter with and react to the extracellular matrix materials on a nanometer scale. Recent advances in nanofabrication technologies allowing the precise control of a nanostructure's pattern, periodicity, shape, and height have enabled a systematic study of cell interactions with three-dimensional nanotopographies. In this report, we examined the behavior of human foreskin fibroblasts on well-ordered dense arrays (post and grate patterns with a 230-nm pitch) of sharp-tip nanostructures with varying three-dimensionalities (from 50 to 600 nm in structural height) over time-until a cell sheet was formed. Although cells started out smaller and proliferated slower on tall nanostructures (both posts and grates) than on smooth surfaces, they became confluent to form a sheet in 3 weeks. On grate patterns, significant cell elongation in alignment with the underlying pattern was observed and maintained over time. On tall nanostructures, cells grew while raised on sharp tips, resulting in a weak total adherence to the solid surface. A sheet of cells was easily peeled off from such surfaces, suggesting that nanoscale topographies can be used as the basis for cell-sheet tissue engineering.
AB - Cells in vivo encounter with and react to the extracellular matrix materials on a nanometer scale. Recent advances in nanofabrication technologies allowing the precise control of a nanostructure's pattern, periodicity, shape, and height have enabled a systematic study of cell interactions with three-dimensional nanotopographies. In this report, we examined the behavior of human foreskin fibroblasts on well-ordered dense arrays (post and grate patterns with a 230-nm pitch) of sharp-tip nanostructures with varying three-dimensionalities (from 50 to 600 nm in structural height) over time-until a cell sheet was formed. Although cells started out smaller and proliferated slower on tall nanostructures (both posts and grates) than on smooth surfaces, they became confluent to form a sheet in 3 weeks. On grate patterns, significant cell elongation in alignment with the underlying pattern was observed and maintained over time. On tall nanostructures, cells grew while raised on sharp tips, resulting in a weak total adherence to the solid surface. A sheet of cells was easily peeled off from such surfaces, suggesting that nanoscale topographies can be used as the basis for cell-sheet tissue engineering.
KW - Cell attachment/ detachment
KW - Cell morphology
KW - Cell proliferation
KW - Cell sheet
KW - Nanotopography
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UR - http://www.scopus.com/inward/citedby.url?scp=66249114354&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.32101
DO - 10.1002/jbm.a.32101
M3 - Article
C2 - 18523950
AN - SCOPUS:66249114354
SN - 1549-3296
VL - 89
SP - 804
EP - 817
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
IS - 3
ER -