TY - JOUR
T1 - Cellulose and collagen derived micro-nano structured scaffolds for bone tissue engineering
AU - Aravamudhan, Aja
AU - Ramos, Daisy M.
AU - Nip, Jonathan
AU - Harmon, Matthew D.
AU - James, Roshan
AU - Deng, Meng
AU - Laurencin, Cato T.
AU - Yu, Xiaojun
AU - Kumbar, Sangamesh G.
PY - 2013/4
Y1 - 2013/4
N2 - Scaffold based bone tissue engineering (BTE) has made great progress in regenerating lost bone tissue. Materials of natural and synthetic origin have been used for scaffold fabrication. Scaffolds derived from natural polymers offer greater bioactivity and biocompatibility with mammalian tissues to favor tissue healing, due to their similarity to native extracellular matrix (ECM) components. Often it is a challenge to fabricate natural polymer based scaffolds for BTE applications without compromising their bioactivity, while maintaining adequate mechanical properties. In this work, we report the fabrication and characterization of cellulose and collagen based micro-nano structured scaffolds using human osteoblasts (HOB) for BTE applications. These porous micro-nano structured scaffolds (average pore diameter 190±10 μm) exhibited mechanical properties in the mid range of human trabecular bone (compressive modulus 266.75±33.22 MPa and strength 12.15± 2.23 MPa). These scaffolds supported the greater adhesion and phenotype maintenance of cultured HOB as reflected by higher levels of osteogenic enzyme alkaline phosphatase and mineral deposition compared to control polyester micro-nano structured scaffolds of identical pore properties. These natural polymer based micro-nano structured scaffolds may serve as alternatives to polyester based scaffolds for BTE applications.
AB - Scaffold based bone tissue engineering (BTE) has made great progress in regenerating lost bone tissue. Materials of natural and synthetic origin have been used for scaffold fabrication. Scaffolds derived from natural polymers offer greater bioactivity and biocompatibility with mammalian tissues to favor tissue healing, due to their similarity to native extracellular matrix (ECM) components. Often it is a challenge to fabricate natural polymer based scaffolds for BTE applications without compromising their bioactivity, while maintaining adequate mechanical properties. In this work, we report the fabrication and characterization of cellulose and collagen based micro-nano structured scaffolds using human osteoblasts (HOB) for BTE applications. These porous micro-nano structured scaffolds (average pore diameter 190±10 μm) exhibited mechanical properties in the mid range of human trabecular bone (compressive modulus 266.75±33.22 MPa and strength 12.15± 2.23 MPa). These scaffolds supported the greater adhesion and phenotype maintenance of cultured HOB as reflected by higher levels of osteogenic enzyme alkaline phosphatase and mineral deposition compared to control polyester micro-nano structured scaffolds of identical pore properties. These natural polymer based micro-nano structured scaffolds may serve as alternatives to polyester based scaffolds for BTE applications.
KW - Cellulose
KW - Collagen
KW - Human Osteoblasts
KW - Micro-Nano Structure
KW - Polysaccharide Bone Grafts
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U2 - 10.1166/jbn.2013.1574
DO - 10.1166/jbn.2013.1574
M3 - Article
C2 - 23621034
AN - SCOPUS:84876521191
SN - 1550-7033
VL - 9
SP - 719
EP - 731
JO - Journal of Biomedical Nanotechnology
JF - Journal of Biomedical Nanotechnology
IS - 4
ER -