TY - JOUR
T1 - Cloning and expression of Staphylococcus aureus and Streptococcus pyogenes murD genes encoding uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligases
AU - El-Sherbeini, Mohamed
AU - Geissler, Wayne M.
AU - Pittman, Jamya
AU - Yuan, Xiling
AU - Wong, Kenny K.
AU - Pompliano, David L.
PY - 1998/3/27
Y1 - 1998/3/27
N2 - Bacterial UDP-N-acetylmuramyl-L-alanine:D-glutamate ligase (MurD), a cytoplasmic peptidoglycan biosynthetic enzyme, catalyzes the ATP-dependent addition of D-glutamate to an alanyl residue of the UDP-N-acetylmuramyl-L-alanine precursor, generating the dipeptide. The murD gene was cloned from both Staphylococcus aureus and Streptococcus pyogenes. Sequence analysis of the S. aureus murD gene revealed an open reading frame of 449 amino acids. The deduced aa sequence of S. aureus MurD is highly homologous to MurD from Escherichia coli, Haemophilus influenzae, Bacillus subtilis and St. pyogenes. Recombinant MurD protein from both S. aureus and St. pyogenes was separately overproduced in E. coli and purified as His-tagged fusion. Both recombinant enzymes catalyzed the ATP-dependent addition of D-glutamate to the precursor sugar peptide.
AB - Bacterial UDP-N-acetylmuramyl-L-alanine:D-glutamate ligase (MurD), a cytoplasmic peptidoglycan biosynthetic enzyme, catalyzes the ATP-dependent addition of D-glutamate to an alanyl residue of the UDP-N-acetylmuramyl-L-alanine precursor, generating the dipeptide. The murD gene was cloned from both Staphylococcus aureus and Streptococcus pyogenes. Sequence analysis of the S. aureus murD gene revealed an open reading frame of 449 amino acids. The deduced aa sequence of S. aureus MurD is highly homologous to MurD from Escherichia coli, Haemophilus influenzae, Bacillus subtilis and St. pyogenes. Recombinant MurD protein from both S. aureus and St. pyogenes was separately overproduced in E. coli and purified as His-tagged fusion. Both recombinant enzymes catalyzed the ATP-dependent addition of D-glutamate to the precursor sugar peptide.
KW - Bacterial cell wall
KW - Gram-positive cocci
KW - Peptidoglycan
UR - http://www.scopus.com/inward/record.url?scp=0032571589&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032571589&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(98)00059-6
DO - 10.1016/S0378-1119(98)00059-6
M3 - Article
C2 - 9524242
AN - SCOPUS:0032571589
SN - 0378-1119
VL - 210
SP - 117
EP - 125
JO - Gene
JF - Gene
IS - 1
ER -