TY - JOUR
T1 - Co-electrospun fibrous scaffold-adsorbed DNA for substrate-mediated gene delivery
AU - Zhang, Jun
AU - Duan, Yajun
AU - Wei, Di
AU - Wang, Lianyong
AU - Wang, Hongjun
AU - Gu, Zhongwei
AU - Kong, Deling
PY - 2011/1
Y1 - 2011/1
N2 - Incorporation of gene into electrospun nanofibers for localized gene transfection of target cells represents a robust platform for tissue regeneration. In this study, a new two-step approach was explored to immobilize DNA onto electrospun nanofibers for effective gene delivery, that is, nonviral gene vector of polyethylene glycol (PEG)-modified polyethylenimine (PEI) was incorporated into scaffolds by electrospinning and then target DNA was adsorbed onto the electrospun nanofibers via electrostatic interaction between DNA and PEI-PEG. PEI-PEG/DNA particles formed from the released DNA, and PEI-PEG had a uniform particle size of approximately 200 nm. This nanofiber-based gene delivery system exhibited high transfection efficiency, in which >65% of human embryonic kidney 293 cells and >40% of mesenchymal stem cells were transfected with green fluorescent protein gene. Compared with PEI, PEG modification of PEI had improved the biocompatibility and further increased the transfection efficiency. These results suggest that the combination of nonviral gene carrier with electrospun nanofibers could be used for localized gene delivery, which has multifold potential applications in tissue engineering or as an in vivo substrate for tissue regeneration.
AB - Incorporation of gene into electrospun nanofibers for localized gene transfection of target cells represents a robust platform for tissue regeneration. In this study, a new two-step approach was explored to immobilize DNA onto electrospun nanofibers for effective gene delivery, that is, nonviral gene vector of polyethylene glycol (PEG)-modified polyethylenimine (PEI) was incorporated into scaffolds by electrospinning and then target DNA was adsorbed onto the electrospun nanofibers via electrostatic interaction between DNA and PEI-PEG. PEI-PEG/DNA particles formed from the released DNA, and PEI-PEG had a uniform particle size of approximately 200 nm. This nanofiber-based gene delivery system exhibited high transfection efficiency, in which >65% of human embryonic kidney 293 cells and >40% of mesenchymal stem cells were transfected with green fluorescent protein gene. Compared with PEI, PEG modification of PEI had improved the biocompatibility and further increased the transfection efficiency. These results suggest that the combination of nonviral gene carrier with electrospun nanofibers could be used for localized gene delivery, which has multifold potential applications in tissue engineering or as an in vivo substrate for tissue regeneration.
KW - electrospun fibers
KW - gene delivery
KW - multifunctional scaffold
KW - polyethylenimine (PEI)
KW - tissue engineering
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U2 - 10.1002/jbm.a.32962
DO - 10.1002/jbm.a.32962
M3 - Article
C2 - 21105170
AN - SCOPUS:78649597597
SN - 1549-3296
VL - 96 A
SP - 212
EP - 220
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
IS - 1
ER -