TY - GEN
T1 - Combining optical coherence tomography with acoustic radiation force for depth-dependent biomechanics of crystalline lens
AU - Wang, Shang
AU - Aglyamov, Salavat
AU - Karpiouk, Andrei
AU - Li, Jiasong
AU - Emelianov, Stanislav
AU - Manns, Fabrice
AU - Larin, Kirill V.
PY - 2013
Y1 - 2013
N2 - Noninvasively probing the biomechanical properties of crystalline lens has been challenging due to its unique features such as location inside the eye and being optically and ultrasonically transparent. Here we introduce a method of relying on the spectral analysis of the lens surface response to a mechanical stimulation for the depthdependent assessment of lens biomechanical properties. In this method, acoustic radiation force (ARF) is used to remotely induce the deformation on the surface of the crystalline lens, and a phase-sensitive optical coherence tomography (PhS-OCT) system, co-focused with ARF, utilized to monitor the localized temporal response of ARFinduced deformations on the lens surface. The dominant frequency from the amplitude spectra of the surface response is obtained as the indicator of the depthwise elasticity distribution. Pilot experiments were performed on tissue-mimicking layered phantoms and ex vivo porcine crystalline lens. Results indicate that the frequency response of the sample surface is contributed by the mechanical properties of layers located at different depths and the depthdependent elastic properties can be revealed from the amplitude spectrum. Further study will be focused on combining the experimental measurements with theoretical model and inverse numerical method for depth-resolved elastography of the crystalline lens.
AB - Noninvasively probing the biomechanical properties of crystalline lens has been challenging due to its unique features such as location inside the eye and being optically and ultrasonically transparent. Here we introduce a method of relying on the spectral analysis of the lens surface response to a mechanical stimulation for the depthdependent assessment of lens biomechanical properties. In this method, acoustic radiation force (ARF) is used to remotely induce the deformation on the surface of the crystalline lens, and a phase-sensitive optical coherence tomography (PhS-OCT) system, co-focused with ARF, utilized to monitor the localized temporal response of ARFinduced deformations on the lens surface. The dominant frequency from the amplitude spectra of the surface response is obtained as the indicator of the depthwise elasticity distribution. Pilot experiments were performed on tissue-mimicking layered phantoms and ex vivo porcine crystalline lens. Results indicate that the frequency response of the sample surface is contributed by the mechanical properties of layers located at different depths and the depthdependent elastic properties can be revealed from the amplitude spectrum. Further study will be focused on combining the experimental measurements with theoretical model and inverse numerical method for depth-resolved elastography of the crystalline lens.
KW - Acoustic radiation force
KW - Crystalline lens
KW - Depth-dependent biomechanics
KW - Optical coherence tomography
KW - Optical phase measurement
KW - Tissue-mimicking layered phantom
UR - http://www.scopus.com/inward/record.url?scp=84896937410&partnerID=8YFLogxK
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U2 - 10.1117/12.2041976
DO - 10.1117/12.2041976
M3 - Conference contribution
AN - SCOPUS:84896937410
SN - 9780819498472
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII
T2 - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII
Y2 - 3 February 2014 through 5 February 2014
ER -