TY - JOUR
T1 - Conditions of lateral surface confinement that promote tissue-cell integration and inhibit biofilm growth
AU - Wang, Yi
AU - da Silva Domingues, Joana F.
AU - Subbiahdoss, Guruprakash
AU - van der Mei, Henny C.
AU - Busscher, Henk J.
AU - Libera, Matthew
PY - 2014/7
Y1 - 2014/7
N2 - Surfaces with cell adhesiveness modulated at micro length scales can exploit differences between tissue/bacterial cell size, membrane/wall plasticity, and adhesion mechanisms to differentially control tissue-cell/material and bacteria/material interactions. This study explores the short-term interactions of Staphylococcus aureus and osteoblast-like cells with surfaces consisting of cell-adhesive circular patches (1-5μm diameter) separated by non-adhesive electron-beam patterned poly(ethylene glycol) hydrogel thin films at inter-patch distances of 0.5-10μm. Osteoblast-like U2OS cells both bind to and spread on the modulated surfaces, in some cases when the cell-adhesive area comprises only 9% of the total surface and in several cases at least as well as on the continuously adhesive control surfaces. In contrast, S.aureus adhesion rates are 7-20 times less on the modulated surfaces than on the control surfaces. Furthermore, the proliferation of those bacteria that do adhere is inhibited by the lateral confinement imposed by the non-adhesive boundaries surrounding each patch. These findings suggest a new approach to create biomaterial surfaces that may promote healing while simultaneously reducing the probability of infection.
AB - Surfaces with cell adhesiveness modulated at micro length scales can exploit differences between tissue/bacterial cell size, membrane/wall plasticity, and adhesion mechanisms to differentially control tissue-cell/material and bacteria/material interactions. This study explores the short-term interactions of Staphylococcus aureus and osteoblast-like cells with surfaces consisting of cell-adhesive circular patches (1-5μm diameter) separated by non-adhesive electron-beam patterned poly(ethylene glycol) hydrogel thin films at inter-patch distances of 0.5-10μm. Osteoblast-like U2OS cells both bind to and spread on the modulated surfaces, in some cases when the cell-adhesive area comprises only 9% of the total surface and in several cases at least as well as on the continuously adhesive control surfaces. In contrast, S.aureus adhesion rates are 7-20 times less on the modulated surfaces than on the control surfaces. Furthermore, the proliferation of those bacteria that do adhere is inhibited by the lateral confinement imposed by the non-adhesive boundaries surrounding each patch. These findings suggest a new approach to create biomaterial surfaces that may promote healing while simultaneously reducing the probability of infection.
KW - Biofilm
KW - Cell adhesion
KW - Infection
KW - Micropatterning
KW - Osteoblast
KW - Polyethylene oxide
UR - http://www.scopus.com/inward/record.url?scp=84899487908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899487908&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2014.03.057
DO - 10.1016/j.biomaterials.2014.03.057
M3 - Article
C2 - 24726539
AN - SCOPUS:84899487908
SN - 0142-9612
VL - 35
SP - 5446
EP - 5452
JO - Biomaterials
JF - Biomaterials
IS - 21
ER -