Conditions of lateral surface confinement that promote tissue-cell integration and inhibit biofilm growth

Yi Wang, Joana F. da Silva Domingues, Guruprakash Subbiahdoss, Henny C. van der Mei, Henk J. Busscher, Matthew Libera

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Surfaces with cell adhesiveness modulated at micro length scales can exploit differences between tissue/bacterial cell size, membrane/wall plasticity, and adhesion mechanisms to differentially control tissue-cell/material and bacteria/material interactions. This study explores the short-term interactions of Staphylococcus aureus and osteoblast-like cells with surfaces consisting of cell-adhesive circular patches (1-5μm diameter) separated by non-adhesive electron-beam patterned poly(ethylene glycol) hydrogel thin films at inter-patch distances of 0.5-10μm. Osteoblast-like U2OS cells both bind to and spread on the modulated surfaces, in some cases when the cell-adhesive area comprises only 9% of the total surface and in several cases at least as well as on the continuously adhesive control surfaces. In contrast, S.aureus adhesion rates are 7-20 times less on the modulated surfaces than on the control surfaces. Furthermore, the proliferation of those bacteria that do adhere is inhibited by the lateral confinement imposed by the non-adhesive boundaries surrounding each patch. These findings suggest a new approach to create biomaterial surfaces that may promote healing while simultaneously reducing the probability of infection.

Original languageEnglish
Pages (from-to)5446-5452
Number of pages7
JournalBiomaterials
Volume35
Issue number21
DOIs
StatePublished - Jul 2014

Keywords

  • Biofilm
  • Cell adhesion
  • Infection
  • Micropatterning
  • Osteoblast
  • Polyethylene oxide

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