TY - JOUR
T1 - Continuous plug-flow bioreactor
T2 - Experimental testing with Pseudomonas putida culture grown on benzoate
AU - Voloshin, Yury
AU - Lawal, Adeniyi
AU - Panikov, Nicolai S.
PY - 2005/7/20
Y1 - 2005/7/20
N2 - The goals of this work were to test the feasibility of a continuous plug-flow (PF) bioreactor and to compare the growth in the PF bioreactor to that in a batch bioreactor. A culture of Pseudomonas putida was pumped through a tube made of Teflon with varying residence times. The culture was aerated by pumping of air simultaneously with liquid medium to provide air bubbles along the tubular culture. When the residence time in the PF bioreactor was greater than the time needed to reach the stationary phase in batch mode, the maximum biomass density reached in PF mode was the same as the maximum density reached in the batch bioreactor, and benzoate (the only carbon and energy source) was completely consumed. The drawbacks for practical application of PF were found to be fluctuations of cell concentration in the outflow cultural liquid due to cell aggregation, significant cell adhesion to the inner wall of Teflon tubing, and inadequate aeration.
AB - The goals of this work were to test the feasibility of a continuous plug-flow (PF) bioreactor and to compare the growth in the PF bioreactor to that in a batch bioreactor. A culture of Pseudomonas putida was pumped through a tube made of Teflon with varying residence times. The culture was aerated by pumping of air simultaneously with liquid medium to provide air bubbles along the tubular culture. When the residence time in the PF bioreactor was greater than the time needed to reach the stationary phase in batch mode, the maximum biomass density reached in PF mode was the same as the maximum density reached in the batch bioreactor, and benzoate (the only carbon and energy source) was completely consumed. The drawbacks for practical application of PF were found to be fluctuations of cell concentration in the outflow cultural liquid due to cell aggregation, significant cell adhesion to the inner wall of Teflon tubing, and inadequate aeration.
KW - Aeration
KW - Growth kinetics
KW - Resilience time
KW - Substrate utilization
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U2 - 10.1002/bit.20521
DO - 10.1002/bit.20521
M3 - Article
C2 - 15895381
AN - SCOPUS:23844543073
SN - 0006-3592
VL - 91
SP - 254
EP - 259
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 2
ER -