DEVELOPMENT OF AN OPEN-SOURCE LOW-COST MODULAR QUAD-EXTRUSION 3D BIOPRINTER

Ralf Zgeib, Xiaofeng Wang, Ahmadreza Zaeri, Fucheng Zhang, Kai Cao, Robert Chang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Advanced additive manufacturing (AM) technologies are being harnessed to capture the complex range and specificity of native tissue properties towards fully functional bioprinted tissue constructs. Such enabling technologies have been reported to recapitulate the complexity and heterogeneity of the native tissues. However, the challenges of cost and scalability hamper broad AM process adoption and implementation for fundamental research in the life sciences as well as for clinical end-use applications. In order to address the cost barrier to AM adoption, an open-source low-cost modular quad-extrusion multi-material 3D bioprinting system is developed herein to enable the fabrication of complex tissue constructs. The developed quad-extrusion bioprinter (QEB) is established with two divergent printing modes, namely in-air printing (IAP) and support bath printing (SBP), using gelatin methacryloyl as a model hydrogel bioink. Bioprinted performance outcomes are then measured for structural fidelity with benchmarking to the computer-aided design models. Moreover, biological outcomes are qualified by way of a LIVE/DEAD cell viability assay over a 3-day time course. In summary, the developed QEB is shown to be a robust platform that enables the scalable fabrication of multi-material complex tissue constructs at an accessible cost under $300, further closing the gap between developmental and clinical AM platforms.

Original languageEnglish
Title of host publicationAdditive Manufacturing; Advanced Materials Manufacturing; Biomanufacturing; Life Cycle Engineering
ISBN (Electronic)9780791887233
DOIs
StatePublished - 2023
EventASME 2023 18th International Manufacturing Science and Engineering Conference, MSEC 2023 - New Brunswick, United States
Duration: 12 Jun 202316 Jun 2023

Publication series

NameProceedings of ASME 2023 18th International Manufacturing Science and Engineering Conference, MSEC 2023
Volume1

Conference

ConferenceASME 2023 18th International Manufacturing Science and Engineering Conference, MSEC 2023
Country/TerritoryUnited States
CityNew Brunswick
Period12/06/2316/06/23

Keywords

  • 3D Bioprinting
  • Additive Manufacturing
  • GelMA
  • Hydrogels
  • Laponite B
  • Multi-material
  • Quad-extrusion
  • Support Bath Printing

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