Abstract
Hepatocytes cultured on collagen-coated surfaces formed aggregates in William's E medium when supplemented with 1-2% dimethyl sulfoxide (DMSO) without causing obvious cellular injury. Hepatocytes in aggregates remained alive and differentiated for more than one month. DMSO decreased the adhesion of hepatocytes to collagen-coated surfaces which may explain the formation of hepatocyte aggregates.
Original language | English |
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Pages (from-to) | 869-872 |
Number of pages | 4 |
Journal | Biotechnology Techniques |
Volume | 11 |
Issue number | 12 |
DOIs | |
State | Published - 1997 |