Dimethyl sulfoxide induces multilayer aggregates and prolongs survival of primary cultured hepatocytes

J. F. Liang, T. Akaike

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Hepatocytes cultured on collagen-coated surfaces formed aggregates in William's E medium when supplemented with 1-2% dimethyl sulfoxide (DMSO) without causing obvious cellular injury. Hepatocytes in aggregates remained alive and differentiated for more than one month. DMSO decreased the adhesion of hepatocytes to collagen-coated surfaces which may explain the formation of hepatocyte aggregates.

Original languageEnglish
Pages (from-to)869-872
Number of pages4
JournalBiotechnology Techniques
Volume11
Issue number12
DOIs
StatePublished - 1997

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