Dimethyl sulfoxide induces multilayer aggregates and prolongs survival of primary cultured hepatocytes

J. F. Liang; T. Akaike

doi:10.1023/A:1018402014414

Dimethyl sulfoxide induces multilayer aggregates and prolongs survival of primary cultured hepatocytes

J. F. Liang
, T. Akaike

Department of Chemistry and Chemical Biology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Hepatocytes cultured on collagen-coated surfaces formed aggregates in William's E medium when supplemented with 1-2% dimethyl sulfoxide (DMSO) without causing obvious cellular injury. Hepatocytes in aggregates remained alive and differentiated for more than one month. DMSO decreased the adhesion of hepatocytes to collagen-coated surfaces which may explain the formation of hepatocyte aggregates.

Original language	English
Pages (from-to)	869-872
Number of pages	4
Journal	Biotechnology Techniques
Volume	11
Issue number	12
DOIs	https://doi.org/10.1023/A:1018402014414
State	Published - 1997

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title = "Dimethyl sulfoxide induces multilayer aggregates and prolongs survival of primary cultured hepatocytes",

abstract = "Hepatocytes cultured on collagen-coated surfaces formed aggregates in William's E medium when supplemented with 1-2\% dimethyl sulfoxide (DMSO) without causing obvious cellular injury. Hepatocytes in aggregates remained alive and differentiated for more than one month. DMSO decreased the adhesion of hepatocytes to collagen-coated surfaces which may explain the formation of hepatocyte aggregates.",

author = "Liang, \{J. F.\} and T. Akaike",

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T1 - Dimethyl sulfoxide induces multilayer aggregates and prolongs survival of primary cultured hepatocytes

AU - Liang, J. F.

AU - Akaike, T.

PY - 1997

Y1 - 1997

N2 - Hepatocytes cultured on collagen-coated surfaces formed aggregates in William's E medium when supplemented with 1-2% dimethyl sulfoxide (DMSO) without causing obvious cellular injury. Hepatocytes in aggregates remained alive and differentiated for more than one month. DMSO decreased the adhesion of hepatocytes to collagen-coated surfaces which may explain the formation of hepatocyte aggregates.

AB - Hepatocytes cultured on collagen-coated surfaces formed aggregates in William's E medium when supplemented with 1-2% dimethyl sulfoxide (DMSO) without causing obvious cellular injury. Hepatocytes in aggregates remained alive and differentiated for more than one month. DMSO decreased the adhesion of hepatocytes to collagen-coated surfaces which may explain the formation of hepatocyte aggregates.

UR - https://www.scopus.com/pages/publications/0031472218

UR - https://www.scopus.com/pages/publications/0031472218#tab=citedBy

U2 - 10.1023/A:1018402014414

DO - 10.1023/A:1018402014414

M3 - Article

AN - SCOPUS:0031472218

SN - 0951-208X

VL - 11

SP - 869

EP - 872

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 12

ER -

Dimethyl sulfoxide induces multilayer aggregates and prolongs survival of primary cultured hepatocytes

Abstract

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