TY - JOUR
T1 - Dose-dependent modulation effects of bioactive glass particles on macrophages and diabetic wound healing
AU - Xie, Weihan
AU - Fu, Xiaoling
AU - Tang, Fengling
AU - Mo, Yunfei
AU - Cheng, Jun
AU - Wang, Hongjun
AU - Chen, Xiaofeng
N1 - Publisher Copyright:
© 2018 The Royal Society of Chemistry.
PY - 2019
Y1 - 2019
N2 - Many pathophysiologic conditions can interrupt the normal wound healing process and lead to chronic wounds due to the arrest of macrophages in their inflammatory phenotype. Thus, strategies that promote the recovery of macrophage functions are of great benefit to heal chronic wounds. Bioactive glass (BG) dissolution has been recognized for its modulatory functions in macrophage polarization. However, further efforts are greatly needed to address how BG particles affect macrophage behaviors (such as proliferation, viability, migration and polarization) and wound healing through both direct contact and released ions. Our results showed that BG particles affect the proliferation/viability and polarization of macrophages in a dose-dependent manner. At a low concentration (20 μg mL -1 ), BG particles stimulated macrophage proliferation and promoted the M1-to-M2 phenotype switch; meanwhile, at a high concentration (100 μg mL -1 ), the particles showed significant cytotoxicity and prolonged the M1 phenotype of macrophages. The BG particles also exhibited strong chemotaxis to macrophages which appeared to be independent of their concentration. Dose-dependent regulation of macrophages and wound closure by BG particles were also observed in the healing of full-thickness wounds of diabetic rats. Our study suggests that the BG particle-mediated activities of macrophages are essential to wound healing, and these activities are greatly correlated with the amount of BG particles.
AB - Many pathophysiologic conditions can interrupt the normal wound healing process and lead to chronic wounds due to the arrest of macrophages in their inflammatory phenotype. Thus, strategies that promote the recovery of macrophage functions are of great benefit to heal chronic wounds. Bioactive glass (BG) dissolution has been recognized for its modulatory functions in macrophage polarization. However, further efforts are greatly needed to address how BG particles affect macrophage behaviors (such as proliferation, viability, migration and polarization) and wound healing through both direct contact and released ions. Our results showed that BG particles affect the proliferation/viability and polarization of macrophages in a dose-dependent manner. At a low concentration (20 μg mL -1 ), BG particles stimulated macrophage proliferation and promoted the M1-to-M2 phenotype switch; meanwhile, at a high concentration (100 μg mL -1 ), the particles showed significant cytotoxicity and prolonged the M1 phenotype of macrophages. The BG particles also exhibited strong chemotaxis to macrophages which appeared to be independent of their concentration. Dose-dependent regulation of macrophages and wound closure by BG particles were also observed in the healing of full-thickness wounds of diabetic rats. Our study suggests that the BG particle-mediated activities of macrophages are essential to wound healing, and these activities are greatly correlated with the amount of BG particles.
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U2 - 10.1039/c8tb02938e
DO - 10.1039/c8tb02938e
M3 - Article
C2 - 32255099
AN - SCOPUS:85061279610
SN - 2050-7518
VL - 7
SP - 940
EP - 952
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 6
ER -