TY - JOUR
T1 - Dual chemical barriers protect a plant against different larval stages of an insect
AU - Renwick, J. Alan A.
AU - Zhang, Wenqing
AU - Haribal, Meena
AU - Attygalle, Athula B.
AU - Lopez, Kimberly D.
PY - 2001
Y1 - 2001
N2 - The host plants of the native American butterfly, Pieris napi oleracea, include most wild mustards. However, garlic mustard, Alliaria petiolata, a highly invasive weed that was introduced from Europe, appears to be protected from this insect. Although adults will oviposit on the plant, most larvae of P. n. oleracea do not survive on garlic mustard. We used feeding bioassays with different larval stages of the insect to monitor the isolation and identification of two bioactive constituents that could explain the natural resistance of this plant. A novel cyanopropenyl glycoside (1), alliarinoside, strongly inhibits feeding by first instars, while a flavone glycoside (2), isovitexin-6″-D-β-glucopyranoside, deters later instars from feeding. Interestingly, the first instars are insensitive to 2, and the late instars are little affected by 1. Furthermore, differential effects of dietary experience on insect responses suggest that 1 acts through a mechanism of post-ingestive inhibition, whereas 2 involves gustatory deterrence of feeding.
AB - The host plants of the native American butterfly, Pieris napi oleracea, include most wild mustards. However, garlic mustard, Alliaria petiolata, a highly invasive weed that was introduced from Europe, appears to be protected from this insect. Although adults will oviposit on the plant, most larvae of P. n. oleracea do not survive on garlic mustard. We used feeding bioassays with different larval stages of the insect to monitor the isolation and identification of two bioactive constituents that could explain the natural resistance of this plant. A novel cyanopropenyl glycoside (1), alliarinoside, strongly inhibits feeding by first instars, while a flavone glycoside (2), isovitexin-6″-D-β-glucopyranoside, deters later instars from feeding. Interestingly, the first instars are insensitive to 2, and the late instars are little affected by 1. Furthermore, differential effects of dietary experience on insect responses suggest that 1 acts through a mechanism of post-ingestive inhibition, whereas 2 involves gustatory deterrence of feeding.
KW - Alliaria
KW - Crucifer
KW - Feeding deterrent
KW - Feeding inhibition
KW - Flavonoid
KW - Garlic mustard
KW - Pieridae
KW - Pieris
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UR - http://www.scopus.com/inward/citedby.url?scp=0034901175&partnerID=8YFLogxK
U2 - 10.1023/A:1010402107427
DO - 10.1023/A:1010402107427
M3 - Article
C2 - 11521397
AN - SCOPUS:0034901175
SN - 0098-0331
VL - 27
SP - 1575
EP - 1583
JO - Journal of Chemical Ecology
JF - Journal of Chemical Ecology
IS - 8
ER -