TY - JOUR
T1 - Dynamic imaging and quantitative analysis of cranial neural tube closure in the mouse embryo using optical coherence tomography
AU - Wang, Shang
AU - Garcia, Monica D.
AU - Lopez, Andrew L.
AU - Overbeek, Paul A.
AU - Larin, Kirill V.
AU - Larina, Irina V.
N1 - Publisher Copyright:
© 2016 Optical Society of America.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Neural tube closure is a critical feature of central nervous system morphogenesis during embryonic development. Failure of this process leads to neural tube defects, one of the most common forms of human congenital defects. Although molecular and genetic studies in model organisms have provided insights into the genes and proteins that are required for normal neural tube development, complications associated with live imaging of neural tube closure in mammals limit efficient morphological analyses. Here, we report the use of optical coherence tomography (OCT) for dynamic imaging and quantitative assessment of cranial neural tube closure in live mouse embryos in culture. Through time-lapse imaging, we captured two neural tube closure mechanisms in different cranial regions, zipper-like closure of the hindbrain region and button-like closure of the midbrain region. We also used OCT imaging for phenotypic characterization of a neural tube defect in a mouse mutant. These results suggest that the described approach is a useful tool for live dynamic analysis of normal neural tube closure and neural tube defects in the mouse model.
AB - Neural tube closure is a critical feature of central nervous system morphogenesis during embryonic development. Failure of this process leads to neural tube defects, one of the most common forms of human congenital defects. Although molecular and genetic studies in model organisms have provided insights into the genes and proteins that are required for normal neural tube development, complications associated with live imaging of neural tube closure in mammals limit efficient morphological analyses. Here, we report the use of optical coherence tomography (OCT) for dynamic imaging and quantitative assessment of cranial neural tube closure in live mouse embryos in culture. Through time-lapse imaging, we captured two neural tube closure mechanisms in different cranial regions, zipper-like closure of the hindbrain region and button-like closure of the midbrain region. We also used OCT imaging for phenotypic characterization of a neural tube defect in a mouse mutant. These results suggest that the described approach is a useful tool for live dynamic analysis of normal neural tube closure and neural tube defects in the mouse model.
KW - Medical and biological imaging
KW - Optical coherence tomography
KW - Time-resolved imaging
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U2 - 10.1364/BOE.8.000407
DO - 10.1364/BOE.8.000407
M3 - Article
AN - SCOPUS:85008953581
VL - 8
SP - 407
EP - 419
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 1
M1 - #280968
ER -