Effect of adsorbed fibronectin on the differential adhesion of osteoblast-like cells and Staphylococcus aureus with and without fibronectin-binding proteins

Y. Wang, G. Subbiahdoss, J. de Vries, M. Libera, H. C. van der Mei, H. J. Busscher

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The influence of fibronectin (Fn) coated surfaces patterned with poly(ethylene glycol) microgels having inter-gel spacings between 0.5 and 3.0 μm on the adhesion of Staphylococcus aureus strains with and without Fn-binding proteins and cellular adhesion/spreading was investigated. Quantitative force measurements between a S. aureus cell and a patterned surface showed that the adhesion force between the bacterium and the patterned surface increased substantially after Fn adsorption, regardless of the strain used, but decreased with decreasing inter-gel spacing. In flow-chamber experiments, the Fn-binding strain adhered at a higher rate after Fn adsorption than the strain lacking Fn-binding proteins. In both cases, the adhesion rates decreased with decreasing inter-gel spacing. Osteoblast-like cells could bind to patterned surfaces despite the microgels, and adsorbed Fn substantially amplified this effect. Even under highly non-adhesive conditions associated with closely spaced microgels, adsorbed Fn preserves a window of inter-gel spacing around 1 μm where the adhesion of staphylococcal cells is hindered while cells can still adhere and spread.

Original languageEnglish
Pages (from-to)1011-1021
Number of pages11
JournalBiofouling
Volume28
Issue number9
DOIs
StatePublished - Sep 2012

Keywords

  • PEG
  • bacterial adhesion
  • cell-material interactions
  • hydrogel
  • patterning, infection

Fingerprint

Dive into the research topics of 'Effect of adsorbed fibronectin on the differential adhesion of osteoblast-like cells and Staphylococcus aureus with and without fibronectin-binding proteins'. Together they form a unique fingerprint.

Cite this