TY - JOUR
T1 - Effects of disordered hemispherical micropatterns on Staphylococcus epidermidis biofilm formation
AU - Ihnen, Andrew C.
AU - Lee, Joung Hyun
AU - Lee, Woo Y.
PY - 2010/2/1
Y1 - 2010/2/1
N2 - Surfaces which have physical patterns in the scale of bacteria cells have been shown to influence the microorganism's adhesion and biofilm formation characteristics. Layer-by-layer self-assembly was utilized to create disordered hemispherical patterns on poly(dimethylsiloxane) with a feature size of 0.5 μm, 1.0 μm and 2.0 μm. The effects of pattern size on the retention and biofilm formation of Staphylococcus epidermidis were examined as a function of culture time. The 1.0 μm pattern significantly reduced biofilm surface coverage by ∼30% after 5 h of culture in comparison to that on an unpatterned surface while the effect of the 0.5 and 2.0 μm patterns was negligible. On the 1.0 μm surface, bacteria initially adhered on the unpatterned areas of the disordered surface and subsequently developed into biofilms by spreading across the unpatterned areas while avoiding those covered by the pattern. The results suggest that the size of surface patterns is an important factor in altering bacteria adhesion and biofilm formation characteristics.
AB - Surfaces which have physical patterns in the scale of bacteria cells have been shown to influence the microorganism's adhesion and biofilm formation characteristics. Layer-by-layer self-assembly was utilized to create disordered hemispherical patterns on poly(dimethylsiloxane) with a feature size of 0.5 μm, 1.0 μm and 2.0 μm. The effects of pattern size on the retention and biofilm formation of Staphylococcus epidermidis were examined as a function of culture time. The 1.0 μm pattern significantly reduced biofilm surface coverage by ∼30% after 5 h of culture in comparison to that on an unpatterned surface while the effect of the 0.5 and 2.0 μm patterns was negligible. On the 1.0 μm surface, bacteria initially adhered on the unpatterned areas of the disordered surface and subsequently developed into biofilms by spreading across the unpatterned areas while avoiding those covered by the pattern. The results suggest that the size of surface patterns is an important factor in altering bacteria adhesion and biofilm formation characteristics.
KW - Biofilm
KW - Infection
KW - Layer-by-layer self-assembly
KW - Staphylococcus epidermidis
KW - Surface patterning
UR - http://www.scopus.com/inward/record.url?scp=70549110012&partnerID=8YFLogxK
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U2 - 10.1016/j.colsurfb.2009.10.014
DO - 10.1016/j.colsurfb.2009.10.014
M3 - Article
C2 - 19892532
AN - SCOPUS:70549110012
SN - 0927-7765
VL - 75
SP - 601
EP - 607
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 2
ER -