@inproceedings{b0174f27486f4c8b8e14e8dad5590798,
title = "Electrokinetically integrated isolation and amplification of protein-binding nucleic acids on a microchip",
abstract = "This paper presents a microchip that integrates the isolation and amplification of target-binding DNA strands in a randomized DNA mixture. Target-binding strands are isolated in the chip via binding with human immunoglobulin E (IgE) immobilized on microbeads, electrophoretically transported through a gel-filled microchannel, captured onto microbead-tethered reverse primers, and amplified using polymerase chain reaction (PCR). Integration of isolation and amplification is achieved using electrophoretic transport through the gel-filled microchannel, which prevents contamination of buffers or reactants while transferring the desired target-binding DNA. Experimental results show that our microchip can isolate and amplify target-binding DNA strands with increased binding affinity to IgE protein.",
keywords = "Affinity binding, Aptamer, Electrophoresis, Microchip, Polymerase chain reaction",
author = "Jinho Kim and Hilton, {John P.} and Yang, {Kyung Ae} and Renjun Pei and Milan Stojanovic and Qiao Lin",
year = "2012",
language = "English",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
pages = "118--120",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}