Electrokinetically integrated isolation and amplification of protein-binding nucleic acids on a microchip

Jinho Kim, John P. Hilton, Kyung Ae Yang, Renjun Pei, Milan Stojanovic, Qiao Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This paper presents a microchip that integrates the isolation and amplification of target-binding DNA strands in a randomized DNA mixture. Target-binding strands are isolated in the chip via binding with human immunoglobulin E (IgE) immobilized on microbeads, electrophoretically transported through a gel-filled microchannel, captured onto microbead-tethered reverse primers, and amplified using polymerase chain reaction (PCR). Integration of isolation and amplification is achieved using electrophoretic transport through the gel-filled microchannel, which prevents contamination of buffers or reactants while transferring the desired target-binding DNA. Experimental results show that our microchip can isolate and amplify target-binding DNA strands with increased binding affinity to IgE protein.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Pages118-120
Number of pages3
StatePublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Affinity binding
  • Aptamer
  • Electrophoresis
  • Microchip
  • Polymerase chain reaction

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