TY - GEN
T1 - Electrokinetically integrated microfluidic isolation and amplification of biomolecule-and cell-binding nucleic acids
AU - Kim, J.
AU - Hilton, J. P.
AU - Yang, K. A.
AU - Pei, R.
AU - Zhu, J.
AU - Stojanovic, M.
AU - Lin, Q.
PY - 2013
Y1 - 2013
N2 - This paper presents electrokinetically based microfluidic integration of isolation and amplification of target-binding nucleic acids. A microfluidic device is used that consists of two microchambers for nucleic acid isolation and amplification connected by a microchannel filled with agarose gel. In the device, target-binding DNA strands are isolated and amplified on surfaces while gel-based electrophoresis is used to transfer the strands between chambers, eliminating the need for complicated flow control components. Experimental results show that the device has the potential to rapidly isolate and amplify nucleic acids in random mixtures against a variety of biological targets, such as biomolecules and cells, with increased binding affinity.
AB - This paper presents electrokinetically based microfluidic integration of isolation and amplification of target-binding nucleic acids. A microfluidic device is used that consists of two microchambers for nucleic acid isolation and amplification connected by a microchannel filled with agarose gel. In the device, target-binding DNA strands are isolated and amplified on surfaces while gel-based electrophoresis is used to transfer the strands between chambers, eliminating the need for complicated flow control components. Experimental results show that the device has the potential to rapidly isolate and amplify nucleic acids in random mixtures against a variety of biological targets, such as biomolecules and cells, with increased binding affinity.
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U2 - 10.1109/MEMSYS.2013.6474418
DO - 10.1109/MEMSYS.2013.6474418
M3 - Conference contribution
AN - SCOPUS:84875468435
SN - 9781467356558
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 1007
EP - 1010
BT - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
T2 - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Y2 - 20 January 2013 through 24 January 2013
ER -