Electrokinetically integrated microfluidic isolation and amplification of biomolecule-and cell-binding nucleic acids

J. Kim, J. P. Hilton, K. A. Yang, R. Pei, J. Zhu, M. Stojanovic, Q. Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

This paper presents electrokinetically based microfluidic integration of isolation and amplification of target-binding nucleic acids. A microfluidic device is used that consists of two microchambers for nucleic acid isolation and amplification connected by a microchannel filled with agarose gel. In the device, target-binding DNA strands are isolated and amplified on surfaces while gel-based electrophoresis is used to transfer the strands between chambers, eliminating the need for complicated flow control components. Experimental results show that the device has the potential to rapidly isolate and amplify nucleic acids in random mixtures against a variety of biological targets, such as biomolecules and cells, with increased binding affinity.

Original languageEnglish
Title of host publicationIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages1007-1010
Number of pages4
DOIs
StatePublished - 2013
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 20 Jan 201324 Jan 2013

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

ConferenceIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Country/TerritoryTaiwan, Province of China
CityTaipei
Period20/01/1324/01/13

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