Abstract
This Letter describes how the endosomal organization of immunostimulatory nanoconstructs can tune the in vitro activation of macrophages. Nanoconstructs composed of gold nanoparticles conjugated with unmethylated cytosine-phosphate-guanine (CpG) oligonucleotides have distinct endosomal distributions depending on the surface curvature. Mixed-curvature constructs produce a relatively high percentage of hollow endosomes, where constructs accumulated primarily along the interior edges. These constructs achieved a higher level of toll-like receptor (TLR) 9 activation along with the enhanced secretion of proinflammatory cytokines and chemokines compared to constant-curvature constructs that aggregated mostly in the center of the endosomes. Our results underscore the importance of intraendosomal interactions in regulating immune responses and targeted delivery.
Original language | English |
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Pages (from-to) | 6170-6175 |
Number of pages | 6 |
Journal | Nano Letters |
Volume | 20 |
Issue number | 8 |
DOIs | |
State | Published - 12 Aug 2020 |
Keywords
- CpG
- immunostimulation
- intracellular targeting
- nanoparticle
- surface curvature