TY - JOUR
T1 - Enhancement of Schwann Cells Function Using Graphene-Oxide-Modified Nanofiber Scaffolds for Peripheral Nerve Regeneration
AU - Wang, Juan
AU - Zheng, Wei
AU - Chen, Liang
AU - Zhu, Tonghe
AU - Shen, Wei
AU - Fan, Cunyi
AU - Wang, Hongjun
AU - Mo, Xiumei
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019
Y1 - 2019
N2 - Peripheral nerve regeneration and functional recovery remain a significant clinical challenge. Biomaterials that can regulate biological behavior of Schwann cell (SC) and promote neural cell differentiation are beneficial for nerve regeneration and functional recovery. Graphene oxide (GO), as a bioactive nanomaterial, has attracted great attention in biomedical applications. In this study, the possibility of combining the excellent physicochemical properties of GO with nanofiber to develop a bioactive scaffold for nerve regeneration were explored. Briefly, GO was coated on the ApF/PLCL scaffolds. To demonstrate the potentials of this platform, in vitro and in vivo studies toward nerve regeneration were carried out. In vitro, GO-coated scaffolds could enhance SC biological behaviors including migration, proliferation, and myelination. The secretions from SCs cultured on GO-ApF/PLCL scaffolds could induce PC12 cells differentiation. Furthermore, GO-coated nanofibrous scaffolds proved to up-regulate focal adhesion kinase (FAK) expression of PC12 cell. In vivo, GO-ApF/PLCL nerve conduits could successfully repair a 10 mm sciatic nerve defect. These findings suggest that GO-based scaffolds efficiently modulate cell functions and promote nerve regeneration, indicating their potential for nerve regeneration applications.
AB - Peripheral nerve regeneration and functional recovery remain a significant clinical challenge. Biomaterials that can regulate biological behavior of Schwann cell (SC) and promote neural cell differentiation are beneficial for nerve regeneration and functional recovery. Graphene oxide (GO), as a bioactive nanomaterial, has attracted great attention in biomedical applications. In this study, the possibility of combining the excellent physicochemical properties of GO with nanofiber to develop a bioactive scaffold for nerve regeneration were explored. Briefly, GO was coated on the ApF/PLCL scaffolds. To demonstrate the potentials of this platform, in vitro and in vivo studies toward nerve regeneration were carried out. In vitro, GO-coated scaffolds could enhance SC biological behaviors including migration, proliferation, and myelination. The secretions from SCs cultured on GO-ApF/PLCL scaffolds could induce PC12 cells differentiation. Furthermore, GO-coated nanofibrous scaffolds proved to up-regulate focal adhesion kinase (FAK) expression of PC12 cell. In vivo, GO-ApF/PLCL nerve conduits could successfully repair a 10 mm sciatic nerve defect. These findings suggest that GO-based scaffolds efficiently modulate cell functions and promote nerve regeneration, indicating their potential for nerve regeneration applications.
KW - ApF/PLCL nanofibrous scaffolds
KW - FAK
KW - GO
KW - SC biological behaviors
KW - peripheral nerve regeneration
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U2 - 10.1021/acsbiomaterials.8b01564
DO - 10.1021/acsbiomaterials.8b01564
M3 - Article
AN - SCOPUS:85065527524
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
ER -