TY - JOUR
T1 - Examining lysozyme structures on polyzwitterionic brush surfaces
AU - Wang, Haoyu
AU - Akcora, Pinar
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Conformational structures of lysozyme at the interfaces of hydrophilic polymer poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), are examined to understand the role of protein-polymer interactions on the stability of lysozyme. This work underpins the effect of hydration layer on the structures of physically adsorbed lysozyme on PMEDSAH brushes. Hydrophilic nature and strength of hydration layers around brushes are controlled by varying the brush thickness and temperature. We measured that lysozyme is structurally less stable on 15 nm thick hydrophilic PMEDSAH brushes at 75 °C than at room temperature. To the contrary, 5–8 nm thick brushes stretch in hydrated state by heating, hence yield higher structural stability of lysozyme. These results suggest that short polyzwitterionic brushes can facilitate improved biomaterial interactions that are essential for biosensors performing at elevated temperatures.
AB - Conformational structures of lysozyme at the interfaces of hydrophilic polymer poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), are examined to understand the role of protein-polymer interactions on the stability of lysozyme. This work underpins the effect of hydration layer on the structures of physically adsorbed lysozyme on PMEDSAH brushes. Hydrophilic nature and strength of hydration layers around brushes are controlled by varying the brush thickness and temperature. We measured that lysozyme is structurally less stable on 15 nm thick hydrophilic PMEDSAH brushes at 75 °C than at room temperature. To the contrary, 5–8 nm thick brushes stretch in hydrated state by heating, hence yield higher structural stability of lysozyme. These results suggest that short polyzwitterionic brushes can facilitate improved biomaterial interactions that are essential for biosensors performing at elevated temperatures.
KW - Biointerface
KW - Hydration layer
KW - Lysozyme
KW - PMEDSAH
KW - Polyzwitterionic brush
KW - Protein structure
KW - Stability
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U2 - 10.1016/j.colsurfb.2017.09.040
DO - 10.1016/j.colsurfb.2017.09.040
M3 - Article
C2 - 28938231
AN - SCOPUS:85029478641
SN - 0927-7765
VL - 160
SP - 215
EP - 219
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -