Fabrication of chitosan films and their biocompatibility to human neofetus dermal and rat dermal fibroblasts

Some chitosan films were fabricated by casting method using glycerol as plasticity agent in dilute acetic acid solution and then characterized. Rat dermal fibrobalasts and human neofetus dermal fibroblasts were cultured in vitro to evaluate the biocompatibility of the chitosan films. The results showed that some cells cold be broken at early stage of the culture because there were too many basic amino groups in chitosan which would tightly bind the negative surface of cells. However, while the culture was continued, cells could normally grow and proliferated. It was found that new cells would be inhibited by contact to each other after they were spread all over the surface of the films. In addition, it was also observed that human neofetus dermal fibroblasts grew slower than rat dermal fibroblasts.