@inproceedings{eb1ef343e70a4221acc66dfd9f96ad71,
title = "Functional optical coherence tomography for high-resolution mapping of cilia beat frequency in the mouse oviduct in vivo",
abstract = "Since mouse is a superior model for genetic analysis of human disorders, reproductive studies in mice have significant implications on further understanding of fertility and infertility in humans. Fertilized oocytes are transported through the reproductive tract by motile cilia lining the lumen of the oviduct as well as by oviduct contractions. While the role of cilia is well recognized, ciliary dynamics in the oviduct is not well understood, largely owing to the lack of live imaging approaches. Here, we report in vivo micro-scale mapping of cilia and cilia beat frequency (CBF) in the mouse oviduct using optical coherence tomography (OCT). This functional imaging method is based on spectral analysis of the OCT speckle variations produced by the beat of cilia in the oviduct, which does not require exogenous contrast agents. Animal procedures similar to the ones used for production of transgenic mice are utilized to expose the reproductive organs for imaging in anesthetized females. In this paper, we first present in vivo structural imaging of the mouse oviduct capturing the oocyte and the preimplantation embryo and then show the result of depth-resolved high-resolution CBF mapping in the ampulla of the live mouse. These data indicate that this structural and functional OCT imaging approach can be a useful tool for a variety of live investigations of mammalian reproduction and infertility.",
keywords = "cilia beat frequency, cumulus cells, functional imaging, in vivo, infertility, mammalian reproduction, mouse oviduct, oocyte, optical coherence tomography, speckles",
author = "Shang Wang and Burton, {Jason C.} and Behringer, {Richard R.} and Larina, {Irina V.}",
note = "Publisher Copyright: {\textcopyright} 2016 SPIE.; Photonic Therapeutics and Diagnostics XII ; Conference date: 13-02-2016 Through 14-02-2016",
year = "2016",
doi = "10.1117/12.2210758",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
editor = "Andreas Mandelis and Bernard Choi and Wong, {Brian J. F.} and Ilgner, {Justus F.} and Laura Marcu and Skala, {Melissa C.} and Nikiforos Kollias and Haishan Zeng and Kang, {Hyun Wook} and Tearney, {Guillermo J.} and Gregory, {Kenton W.} and Campagnola, {Paul J.}",
booktitle = "Photonic Therapeutics and Diagnostics XII",
}