TY - JOUR
T1 - Genetic analysis of the human immunodeficiency virus type 1 integrase protein
AU - Shin, Cha Gyun
AU - Taddeo, Brunella
AU - Haseltine, William A.
AU - Farnet, Chris M.
PY - 1994/3
Y1 - 1994/3
N2 - Single-amino-acid changes in a highly conserved central region of the human immunodeficiency virus type 1 (HIV-1) integrase protein were analyzed for their effects on viral protein synthesis, virion morphogenesis, and viral replication. Alteration of two amino acids that are invariant among retroviral integrases, D116 and E152 of HIV-1, as well as a mutation of the highly conserved amino acid S147 blocked viral replication in two CD4+ human T-cell lines. Mutations of four other highly conserved amino acids in the region had no detectable effect on viral replication, whereas mutations at two positions, N117 and Y143, resulted in viruses with a delayed-replication phenotype. Defects in virion precursor polypeptide processing, virion morphology, or viral DNA synthesis were observed for all of the replication- defective mutants, indicating that changes in integrase can have pleiotropic effects on viral replication.
AB - Single-amino-acid changes in a highly conserved central region of the human immunodeficiency virus type 1 (HIV-1) integrase protein were analyzed for their effects on viral protein synthesis, virion morphogenesis, and viral replication. Alteration of two amino acids that are invariant among retroviral integrases, D116 and E152 of HIV-1, as well as a mutation of the highly conserved amino acid S147 blocked viral replication in two CD4+ human T-cell lines. Mutations of four other highly conserved amino acids in the region had no detectable effect on viral replication, whereas mutations at two positions, N117 and Y143, resulted in viruses with a delayed-replication phenotype. Defects in virion precursor polypeptide processing, virion morphology, or viral DNA synthesis were observed for all of the replication- defective mutants, indicating that changes in integrase can have pleiotropic effects on viral replication.
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U2 - 10.1128/jvi.68.3.1633-1642.1994
DO - 10.1128/jvi.68.3.1633-1642.1994
M3 - Article
C2 - 8107224
AN - SCOPUS:0028006533
SN - 0022-538X
VL - 68
SP - 1633
EP - 1642
JO - Journal of Virology
JF - Journal of Virology
IS - 3
ER -