TY - JOUR
T1 - Gradient Biomolecular Immobilization of 3D Structured Poly-ɛ-caprolactone Biomaterials toward Functional Engineered Tissue
AU - Zaeri, Ahmadreza
AU - Zgeib, Ralf
AU - Zhang, Fucheng
AU - Cao, Kai
AU - Chang, Robert C.
N1 - Publisher Copyright:
© 2023 The Authors. Macromolecular Materials and Engineering published by Wiley-VCH GmbH.
PY - 2023/12
Y1 - 2023/12
N2 - Additive manufacturing (AM) enables the tailored production of precision fibrous scaffolds toward various engineered tissue models. Moreover, by functionalizing scaffolds in either a uniform or gradient pattern of biomolecules, different target tissues can be fabricated in vitro to capture key characteristics of in vivo cellular microenvironments. However, current engineered tissue models lack the appropriate cellular cues that are needed to deterministically direct cell behavior. Specifically, tunable and reproducible scaffold-guided stimuli are identified herein as the missing link between biomaterial structure and cellular behavior. Therefore, the bottleneck of precision control is addressed here over the immobilization of patterned biomolecular stimuli with either uniform or gradient distribution over the AM-enabled 3D biomaterial model as a function of different growth factors exposure variables, protocols, and various scaffold architectural design parameters. The produced study outcomes herein will improve the directing and guiding of biological cell attachment and growth direction in the context of scaffold-guided stimuli techniques. Therefore, unprecedented control is presented here over 3D structured biomaterial gradient functionalization and immobilization of biomolecules toward biomimetic tissue architectures.
AB - Additive manufacturing (AM) enables the tailored production of precision fibrous scaffolds toward various engineered tissue models. Moreover, by functionalizing scaffolds in either a uniform or gradient pattern of biomolecules, different target tissues can be fabricated in vitro to capture key characteristics of in vivo cellular microenvironments. However, current engineered tissue models lack the appropriate cellular cues that are needed to deterministically direct cell behavior. Specifically, tunable and reproducible scaffold-guided stimuli are identified herein as the missing link between biomaterial structure and cellular behavior. Therefore, the bottleneck of precision control is addressed here over the immobilization of patterned biomolecular stimuli with either uniform or gradient distribution over the AM-enabled 3D biomaterial model as a function of different growth factors exposure variables, protocols, and various scaffold architectural design parameters. The produced study outcomes herein will improve the directing and guiding of biological cell attachment and growth direction in the context of scaffold-guided stimuli techniques. Therefore, unprecedented control is presented here over 3D structured biomaterial gradient functionalization and immobilization of biomolecules toward biomimetic tissue architectures.
KW - additive manufacturing
KW - functionalization
KW - gradient biomolecular immobilization
KW - growth factors
KW - melt electrohydrodynamic printing
KW - tissue engineering
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U2 - 10.1002/mame.202300040
DO - 10.1002/mame.202300040
M3 - Article
AN - SCOPUS:85173469966
SN - 1438-7492
VL - 308
JO - Macromolecular Materials and Engineering
JF - Macromolecular Materials and Engineering
IS - 12
M1 - 2300040
ER -