TY - JOUR
T1 - Homogeneous Distribution of Exogenous Cells onto De-epithelialized Rat Trachea via Instillation of Cell-Loaded Hydrogel
AU - Chen, Jiawen
AU - Mir, Seyed Mohammad
AU - Pinezich, Meghan R.
AU - O'Neill, John D.
AU - Guenthart, Brandon A.
AU - Bacchetta, Matthew
AU - Vunjak-Novakovic, Gordana
AU - Huang, Sarah X.L.
AU - Kim, Jinho
N1 - Publisher Copyright:
© 2021 American Chemical Society.
PY - 2022/1/10
Y1 - 2022/1/10
N2 - Injured or diseased airway epithelium due to repeated environmental insults or genetic mutations can lead to a functional decline of the lung and incurable lung diseases. Bioengineered airway tissue constructs can facilitate in vitro investigation of human lung diseases and accelerate the development of effective therapeutics. Here, we report robust tissue manipulation modalities that allow: (i) selective removal of the endogenous epithelium of in vitro cultured airway tissues and (ii) spatially uniform distribution and prolonged cultivation of exogenous cells that are implanted topically onto the denuded airway lumen. Results obtained highlight that our approach to airway tissue manipulation can facilitate controlled removal of the airway epithelium and subsequent homogeneous distribution of newly implanted cells. This study can contribute to the creation of innovative tissue engineering methodologies that can facilitate the treatment of lung diseases, such as cystic fibrosis, primary ciliary dyskinesia, and chronic obstructive pulmonary disease.
AB - Injured or diseased airway epithelium due to repeated environmental insults or genetic mutations can lead to a functional decline of the lung and incurable lung diseases. Bioengineered airway tissue constructs can facilitate in vitro investigation of human lung diseases and accelerate the development of effective therapeutics. Here, we report robust tissue manipulation modalities that allow: (i) selective removal of the endogenous epithelium of in vitro cultured airway tissues and (ii) spatially uniform distribution and prolonged cultivation of exogenous cells that are implanted topically onto the denuded airway lumen. Results obtained highlight that our approach to airway tissue manipulation can facilitate controlled removal of the airway epithelium and subsequent homogeneous distribution of newly implanted cells. This study can contribute to the creation of innovative tissue engineering methodologies that can facilitate the treatment of lung diseases, such as cystic fibrosis, primary ciliary dyskinesia, and chronic obstructive pulmonary disease.
KW - bioreactor
KW - cell replacement
KW - hydrogel
KW - imaging
KW - lung disease
KW - tissue-on-a-chip
UR - http://www.scopus.com/inward/record.url?scp=85121146874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85121146874&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.1c01031
DO - 10.1021/acsbiomaterials.1c01031
M3 - Article
C2 - 34874712
AN - SCOPUS:85121146874
VL - 8
SP - 82
EP - 88
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 1
ER -