TY - JOUR
T1 - Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function
AU - Jongwattanapisan, Prapaporn
AU - Terajima, Masahiko
AU - Miguez, Patricia A.
AU - Querido, William
AU - Nagaoka, Hideaki
AU - Sumida, Noriko
AU - Gurysh, Elizabeth Grace
AU - Ainslie, Kristy M.
AU - Pleshko, Nancy
AU - Perera, Lalith
AU - Yamauchi, Mitsuo
N1 - Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the specific domain ("effector") within the BGN core protein that facilitates BMP-2 osteogenic function. Thus, we generated various recombinant and synthetic peptides corresponding to several domains of BGN, and tested their effects on BMP-2 functions in vitro. The results demonstrated that the leucine-rich repeats 2-3 domain (LRR2-3) of BGN significantly enhanced the BMP-2 induced Smad1/5/9 phosphorylation, osteogenic gene expression, and alkaline phosphatase activity in myogenic C2C12 cells. Furthermore, addition of LRR2-3 to osteoblastic MC3T3-E1 cells accelerated in vitro mineralization without compromising the quality of the mineral and matrix. These data indicate that LRR2-3 is, at least in part, responsible for BGN's ability to enhance BMP-2 osteogenic function, and it could be useful for bone tissue regeneration.
AB - We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the specific domain ("effector") within the BGN core protein that facilitates BMP-2 osteogenic function. Thus, we generated various recombinant and synthetic peptides corresponding to several domains of BGN, and tested their effects on BMP-2 functions in vitro. The results demonstrated that the leucine-rich repeats 2-3 domain (LRR2-3) of BGN significantly enhanced the BMP-2 induced Smad1/5/9 phosphorylation, osteogenic gene expression, and alkaline phosphatase activity in myogenic C2C12 cells. Furthermore, addition of LRR2-3 to osteoblastic MC3T3-E1 cells accelerated in vitro mineralization without compromising the quality of the mineral and matrix. These data indicate that LRR2-3 is, at least in part, responsible for BGN's ability to enhance BMP-2 osteogenic function, and it could be useful for bone tissue regeneration.
UR - https://www.scopus.com/pages/publications/85046658445
UR - https://www.scopus.com/pages/publications/85046658445#tab=citedBy
U2 - 10.1038/s41598-018-25279-x
DO - 10.1038/s41598-018-25279-x
M3 - Article
C2 - 29728612
AN - SCOPUS:85046658445
VL - 8
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 7022
ER -