TY - JOUR
T1 - Identification of three novel peptides isolated from the venom of the neotropical social wasp Polistes major major
AU - Čeřovský, Václav
AU - Pohl, Jan
AU - Yang, Zhihua
AU - Alam, Naseer
AU - Attygalle, Athula B.
PY - 2007/7
Y1 - 2007/7
N2 - Three novel peptides designated as PMM1, PMM2, and PMM3 were isolated and characterized from the venom of the social wasp Polistes major major, one of the most common wasps in the Dominican Republic. By Edman degradation, and MALDI-TOF and ESI-QTOF mass spectrometry, the primary sequences of these peptides were established as follows: PMM1, H-Lys-Arg-Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg-OH (1357.77 Da); PMM2, H-Ile-Asn-Trp-Lys-Lys-Ile-Ala-Ser-Ile-Gly-Lys-Glu-Val-Leu-Lys- Ala-Leu-NH2 (1909.19 Da); and PNM3, H-Phe-Leu-Ser-Ala-Leu- Leu-Gly-Met-Leu-Lys-Asn-Leu-NH2 (1317.78 Da). The suggested sequences were confirmed by MS analysis of peptide fragments obtained by enzymatic digestion. The peptide PMM1 is a lysyl-arginyl-Thr6-bradykinine that belongs to the wasp kinins group. The sequence of the PMM2 peptide is unique; it resembles somewhat the tetradecapeptide amides of the mastoparan group; however, the chain is extended by three additional amino acid residues. The sequence of PMM3 dodecapeptide is homologous to the peptides of the wasp chemotactic group.
AB - Three novel peptides designated as PMM1, PMM2, and PMM3 were isolated and characterized from the venom of the social wasp Polistes major major, one of the most common wasps in the Dominican Republic. By Edman degradation, and MALDI-TOF and ESI-QTOF mass spectrometry, the primary sequences of these peptides were established as follows: PMM1, H-Lys-Arg-Arg-Pro-Pro-Gly-Phe-Thr-Pro-Phe-Arg-OH (1357.77 Da); PMM2, H-Ile-Asn-Trp-Lys-Lys-Ile-Ala-Ser-Ile-Gly-Lys-Glu-Val-Leu-Lys- Ala-Leu-NH2 (1909.19 Da); and PNM3, H-Phe-Leu-Ser-Ala-Leu- Leu-Gly-Met-Leu-Lys-Asn-Leu-NH2 (1317.78 Da). The suggested sequences were confirmed by MS analysis of peptide fragments obtained by enzymatic digestion. The peptide PMM1 is a lysyl-arginyl-Thr6-bradykinine that belongs to the wasp kinins group. The sequence of the PMM2 peptide is unique; it resembles somewhat the tetradecapeptide amides of the mastoparan group; however, the chain is extended by three additional amino acid residues. The sequence of PMM3 dodecapeptide is homologous to the peptides of the wasp chemotactic group.
KW - Chemotactic peptides
KW - Mass spectrometry
KW - Mastoparans
KW - Polistes major major
KW - Wasp kinins
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U2 - 10.1002/psc.860
DO - 10.1002/psc.860
M3 - Article
C2 - 17559065
AN - SCOPUS:34447555959
SN - 1075-2617
VL - 13
SP - 445
EP - 450
JO - Journal of Peptide Science
JF - Journal of Peptide Science
IS - 7
ER -