TY - JOUR
T1 - Impact of 3D hierarchical nanostructures on the antibacterial efficacy of a bacteria-triggered self-defensive antibiotic coating
AU - Hizal, Ferdi
AU - Zhuk, Iryna
AU - Sukhishvili, Svetlana
AU - Busscher, Henk J.
AU - Van Der Mei, Henny C.
AU - Choi, Chang Hwan
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/9/16
Y1 - 2015/9/16
N2 - Titanium is often applied in implant surgery, but frequently implicated in infections associated with bacterial adhesion and growth on the implant surface. Here, we show that hierarchical nanostructuring of titanium and the subsequent coating of resulting topographical features with a self-defensive, antibacterial layer-by-layer (LbL) film enables a synergistic action of hierarchical nanotopography and localized, bacteria-triggered antibiotic release to dramatically enhance the antibacterial efficiency of surfaces. Although sole nanostructuring of titanium substrates did not significantly affect adhesion and growth of Staphylococcus aureus, the coating of 3D-nanopillared substrates with an ultrathin tannic acid/gentamicin (TA/G) LbL film resulted in a 10-fold reduction of the number of surface-attached bacteria. This effect is attributed to the enlarged surface area of the nanostructured coating available for localized bacteria-triggered release of antibiotics, as well as to the lower bacterial adhesion forces resulting in subsided activation of bacterial antibiotic-defense mechanisms when bacteria land on nanopillar tips. The result shows that a combination of 3D nanostructuring with a bacteria-triggered antibiotic-releasing coating presents a unique way to dramatically enhance antibacterial efficacy of biomaterial implants.
AB - Titanium is often applied in implant surgery, but frequently implicated in infections associated with bacterial adhesion and growth on the implant surface. Here, we show that hierarchical nanostructuring of titanium and the subsequent coating of resulting topographical features with a self-defensive, antibacterial layer-by-layer (LbL) film enables a synergistic action of hierarchical nanotopography and localized, bacteria-triggered antibiotic release to dramatically enhance the antibacterial efficiency of surfaces. Although sole nanostructuring of titanium substrates did not significantly affect adhesion and growth of Staphylococcus aureus, the coating of 3D-nanopillared substrates with an ultrathin tannic acid/gentamicin (TA/G) LbL film resulted in a 10-fold reduction of the number of surface-attached bacteria. This effect is attributed to the enlarged surface area of the nanostructured coating available for localized bacteria-triggered release of antibiotics, as well as to the lower bacterial adhesion forces resulting in subsided activation of bacterial antibiotic-defense mechanisms when bacteria land on nanopillar tips. The result shows that a combination of 3D nanostructuring with a bacteria-triggered antibiotic-releasing coating presents a unique way to dramatically enhance antibacterial efficacy of biomaterial implants.
KW - antibacterial coating
KW - bacterial adhesion
KW - biomaterial
KW - nanostructure
KW - titanium
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U2 - 10.1021/acsami.5b05947
DO - 10.1021/acsami.5b05947
M3 - Article
C2 - 26305913
AN - SCOPUS:84941779154
SN - 1944-8244
VL - 7
SP - 20304
EP - 20313
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 36
ER -