TY - GEN
T1 - Improved dialysis technique for core-shell pullulan-polycaprolactone (PCL) nanospheres loaded with hydrophobic ciprofloxacin
AU - Shady-Elghamrawi, Sally F.
AU - Schmidt, Daniel F.
AU - McCarthy, Stephen P.
PY - 2011
Y1 - 2011
N2 - Drug delivery vehicles such as nanospheres improve solubility, bioavailability and biodistribution of many pharmaceutical drugs. Low encapsulation efficiency is a limitation that currently challenges many drug carriers. The dialysis method can be used as a technique to address this problem and enhance the purity and consistency of the materials being prepared. This research demonstrates the encapsulation of hydrophobic ciprofloxacin in Pullulan- Polycaprolactone (PCL) core-shell nanospheres and investigates dialysis times at 24, 48 and 72 hours with and without the addition of ciprofloxacin in the dialysis water. UV spectroscopy showed that the addition of ciprofloxacin to the water used to dialyze loaded nanospheres resulted in the retention of approximately 30-40 times more drugs than the case where loaded nanospheres were dialyzed with pure water. This was also confirmed with release data tested under specified in vitro conditions. The 72 hour groups appeared the most pure, as evidenced by color and texture. Such findings demonstrate an improved dialysis method which can be utilized to enhance drug encapsulation as well as the physical appearance of the loaded nanospheres.
AB - Drug delivery vehicles such as nanospheres improve solubility, bioavailability and biodistribution of many pharmaceutical drugs. Low encapsulation efficiency is a limitation that currently challenges many drug carriers. The dialysis method can be used as a technique to address this problem and enhance the purity and consistency of the materials being prepared. This research demonstrates the encapsulation of hydrophobic ciprofloxacin in Pullulan- Polycaprolactone (PCL) core-shell nanospheres and investigates dialysis times at 24, 48 and 72 hours with and without the addition of ciprofloxacin in the dialysis water. UV spectroscopy showed that the addition of ciprofloxacin to the water used to dialyze loaded nanospheres resulted in the retention of approximately 30-40 times more drugs than the case where loaded nanospheres were dialyzed with pure water. This was also confirmed with release data tested under specified in vitro conditions. The 72 hour groups appeared the most pure, as evidenced by color and texture. Such findings demonstrate an improved dialysis method which can be utilized to enhance drug encapsulation as well as the physical appearance of the loaded nanospheres.
KW - Ciprofloxacin
KW - Dialysis
KW - Nanospheres
KW - Polycaprolactone (PCL)
KW - Pullulan
UR - http://www.scopus.com/inward/record.url?scp=80051821366&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051821366&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:80051821366
SN - 9781617829604
T3 - Annual Technical Conference - ANTEC, Conference Proceedings
SP - 1982
EP - 1988
BT - 69th Annual Technical Conference of the Society of Plastics Engineers 2011, ANTEC 2011
T2 - 69th Annual Technical Conference of the Society of Plastics Engineers 2011, ANTEC 2011
Y2 - 1 May 2011 through 5 May 2011
ER -