Improvement of tissue analysis and classification using optical coherence tomography combined with Raman spectroscopy

Chih Hao Liu, Ji Qi, Jing Lu, Shang Wang, Chen Wu, Wei Chuan Shih, Kirill V. Larin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations

Abstract

Optical coherence tomography (OCT) is an optical imaging technique that is capable of performing high-resolution (approaching the histopathology level) and real-time imaging of tissues without use of contrast agents. Based on these advantages, the pathological features of tumors (in micro scale) can be identified during resection surgery. However, the accuracy of tumor margin prediction still needs to be enhanced for assisting the judgment of surgeons. In this regard, we present a two-dimensional computational method for advanced tissue analysis and characterization based on optical coherence tomography (OCT) and Raman spectroscopy (RS). The method combines the slope of OCT intensity signal and the Principal component (PC) of RS, and relies on the tissue optical attenuation and chemical ingredients for the classification of tissue types. Our pilot experiments were performed on mouse kidney, liver and small intestine. Results demonstrate the improvement of the tissue differentiation compared with the analysis only based on the OCT detection. This combined OCT/RS method is potentially useful as a novel optical biopsy technique for cancer detection.

Original languageEnglish
Title of host publicationDynamics and Fluctuations in Biomedical Photonics XI
DOIs
StatePublished - 2014
EventDynamics and Fluctuations in Biomedical Photonics XI - San Francisco, CA, United States
Duration: 1 Feb 20142 Feb 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8942
ISSN (Print)1605-7422

Conference

ConferenceDynamics and Fluctuations in Biomedical Photonics XI
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/02/142/02/14

Keywords

  • Optical coherence tomography
  • Principle component analysis
  • Raman spectroscopy
  • Support vector machine
  • Tissue characterization

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