TY - CHAP
T1 - In vivo imaging of the mouse reproductive organs, embryo transfer, and oviduct cilia dynamics using optical coherence tomography
AU - Wang, Shang
AU - Larina, Irina V.
N1 - Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2018
Y1 - 2018
N2 - The oviduct (or fallopian tube) serves as the site where a number of major reproductive events occur for the start of a new life in mammals. Understanding the oviduct physiology is essential to uncover hidden mechanisms of the human reproduction and its disorders, yet the current analysis of the oviduct that is largely limited to in vitro imaging is a significant technical hurdle. To overcome this barrier, we have recently developed in vivo approaches based on optical coherence tomography for structural and functional imaging of the mouse oviduct. In this chapter, we describe the details of such live imaging methods that allow for three-dimensional visualization of the oviduct wall morphology, microscale mapping of the oviduct cilia beat frequency, and high-resolution observation of the cumulus–oocyte complex at the cellular level. We expect this set of imaging tools will enable novel studies toward a comprehensive knowledge of the mammalian reproduction.
AB - The oviduct (or fallopian tube) serves as the site where a number of major reproductive events occur for the start of a new life in mammals. Understanding the oviduct physiology is essential to uncover hidden mechanisms of the human reproduction and its disorders, yet the current analysis of the oviduct that is largely limited to in vitro imaging is a significant technical hurdle. To overcome this barrier, we have recently developed in vivo approaches based on optical coherence tomography for structural and functional imaging of the mouse oviduct. In this chapter, we describe the details of such live imaging methods that allow for three-dimensional visualization of the oviduct wall morphology, microscale mapping of the oviduct cilia beat frequency, and high-resolution observation of the cumulus–oocyte complex at the cellular level. We expect this set of imaging tools will enable novel studies toward a comprehensive knowledge of the mammalian reproduction.
KW - Cilia Beat Frequency
KW - Cumulus–Oocyte Complex
KW - In Vivo Imaging
KW - Mammalian Reproduction
KW - Motile Cilia
KW - Mouse Oviduct
KW - Optical Coherence Tomography
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U2 - 10.1007/978-1-4939-7714-7_5
DO - 10.1007/978-1-4939-7714-7_5
M3 - Chapter
C2 - 29564761
AN - SCOPUS:85044244122
T3 - Methods in Molecular Biology
SP - 53
EP - 62
BT - Methods in Molecular Biology
ER -