TY - JOUR
T1 - Influence of systematically varied nano-scale topography on cell morphology and adhesion
AU - Heydarkhan-Hagvall, Sepideh
AU - Choi, Chang Hwan
AU - Dunn, James
AU - Heydarkhan, Sanaz
AU - Schenke-Layland, Katja
AU - MacLellan, W. Robb
AU - Beygui, Ramin
PY - 2007/9
Y1 - 2007/9
N2 - The types of cell-matrix adhesions and the signals they transduce strongly affect the cell-phenotype. We hypothesized that cells sense and respond to the three-dimensionality of their environment, which could be modulated by nano-structures on silicon surfaces. Human foreskin fibroblasts were cultured on nano-structures with different patterns (nano-post and nano-grate) and heights for 3 days. The presence of integrin α5, β1, β3, paxillin and phosphorylated FAK (pFAK) were detected by western blot and immunofluorescence. Integrin β3 exhibited stronger signals on nano-grates. pFAK and paxillin were observed as small dot-like patterns on the cell-periphery on nano-posts and as elongated and aligned patterns on nano-grates. Collectively, our observations highlighted the presence of focal (integrin β1, β3, pFAK, paxillin), fibrillar (integrin α5, β1) and 3-D matrix (integrin α5, β1, paxillin) adhesions on nano-structures. The presented nano-structures offer interesting opportunities to study the interaction of cells with topographical features comparable to the size of extracellular matrix components.
AB - The types of cell-matrix adhesions and the signals they transduce strongly affect the cell-phenotype. We hypothesized that cells sense and respond to the three-dimensionality of their environment, which could be modulated by nano-structures on silicon surfaces. Human foreskin fibroblasts were cultured on nano-structures with different patterns (nano-post and nano-grate) and heights for 3 days. The presence of integrin α5, β1, β3, paxillin and phosphorylated FAK (pFAK) were detected by western blot and immunofluorescence. Integrin β3 exhibited stronger signals on nano-grates. pFAK and paxillin were observed as small dot-like patterns on the cell-periphery on nano-posts and as elongated and aligned patterns on nano-grates. Collectively, our observations highlighted the presence of focal (integrin β1, β3, pFAK, paxillin), fibrillar (integrin α5, β1) and 3-D matrix (integrin α5, β1, paxillin) adhesions on nano-structures. The presented nano-structures offer interesting opportunities to study the interaction of cells with topographical features comparable to the size of extracellular matrix components.
KW - Cell adhesion
KW - Integrins
KW - Nano-structure
UR - http://www.scopus.com/inward/record.url?scp=37849038315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37849038315&partnerID=8YFLogxK
U2 - 10.1080/15419060701755594
DO - 10.1080/15419060701755594
M3 - Article
C2 - 18163229
AN - SCOPUS:37849038315
SN - 1541-9061
VL - 14
SP - 181
EP - 194
JO - Cell Communication and Adhesion
JF - Cell Communication and Adhesion
IS - 5
ER -