TY - JOUR
T1 - Inhibition of lipopolysaccharide and cytokine mixture-mediated hepatocyte nitric oxide synthesis by dimethyl sulfoxide
AU - Liang, Jun F.
AU - Akaike, Toshihiro
PY - 1997/10/20
Y1 - 1997/10/20
N2 - Treatment and pretreatment of hepatocytes with 2% dimethyl sulfoxide (DMSO) inhibited lipopolysaccharide and cytokine mixture (LPS/CM)-mediated NO synthesis in hepatocytes without any obvious effects on cell viability. DMSO at concentrations of 0.5-4% stimulated DNA replication and increased albumin secretion in LPS/CM-treated hepatocytes. Genisein, a inhibitor of protein tyrosine kinase (PTK), inhibited LPS/CM-mediated NO synthesis in hepatocytes. These results suggest that PTK is critical for hepatocyte NO synthesis, and DMSO-inhibited NO synthesis may be associated with prevention of LPS/CM-induced PTK activation in hepatocytes.
AB - Treatment and pretreatment of hepatocytes with 2% dimethyl sulfoxide (DMSO) inhibited lipopolysaccharide and cytokine mixture (LPS/CM)-mediated NO synthesis in hepatocytes without any obvious effects on cell viability. DMSO at concentrations of 0.5-4% stimulated DNA replication and increased albumin secretion in LPS/CM-treated hepatocytes. Genisein, a inhibitor of protein tyrosine kinase (PTK), inhibited LPS/CM-mediated NO synthesis in hepatocytes. These results suggest that PTK is critical for hepatocyte NO synthesis, and DMSO-inhibited NO synthesis may be associated with prevention of LPS/CM-induced PTK activation in hepatocytes.
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U2 - 10.1006/bbrc.1997.7506
DO - 10.1006/bbrc.1997.7506
M3 - Article
C2 - 9344862
AN - SCOPUS:0031581085
SN - 0006-291X
VL - 239
SP - 517
EP - 521
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -