TY - JOUR
T1 - Insights into the Mechanical Characterization of Mouse Brain Tissue Using Microindentation Testing
AU - Zhang, Xuesong
AU - van den Hurk, Eva A.N.
AU - Weickenmeier, Johannes
N1 - Publisher Copyright:
© 2024 Wiley Periodicals LLC.
PY - 2024/4
Y1 - 2024/4
N2 - Indentation testing is the most common approach to quantify mechanical brain tissue properties. Despite a myriad of studies conducted already, reported stiffness values vary extensively and continue to be subject of study. Moreover, the growing interest in the relationship between the brain's spatially heterogeneous microstructure and local tissue stiffness warrants the development of standardized measurement protocols to enable comparability between studies and assess repeatability of reported data. Here, we present three individual protocols that outline (1) sample preparation of a 1000-µm thick coronal slice, (2) a comprehensive list of experimental parameters associated with the FemtoTools FT-MTA03 Micromechanical Testing System for spherical indentation, and (3) two different approaches to derive the elastic modulus from raw force-displacement data. Lastly, we demonstrate that our protocols deliver a robust experimental framework that enables us to determine the spatially heterogeneous microstructural properties of (mouse) brain tissue.
AB - Indentation testing is the most common approach to quantify mechanical brain tissue properties. Despite a myriad of studies conducted already, reported stiffness values vary extensively and continue to be subject of study. Moreover, the growing interest in the relationship between the brain's spatially heterogeneous microstructure and local tissue stiffness warrants the development of standardized measurement protocols to enable comparability between studies and assess repeatability of reported data. Here, we present three individual protocols that outline (1) sample preparation of a 1000-µm thick coronal slice, (2) a comprehensive list of experimental parameters associated with the FemtoTools FT-MTA03 Micromechanical Testing System for spherical indentation, and (3) two different approaches to derive the elastic modulus from raw force-displacement data. Lastly, we demonstrate that our protocols deliver a robust experimental framework that enables us to determine the spatially heterogeneous microstructural properties of (mouse) brain tissue.
KW - brain sample preparation
KW - indentation testing
KW - mechanical characterization
KW - mouse brain tissue
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U2 - 10.1002/cpz1.1011
DO - 10.1002/cpz1.1011
M3 - Article
C2 - 38648070
AN - SCOPUS:85191061559
VL - 4
JO - Current Protocols
JF - Current Protocols
IS - 4
M1 - e1011
ER -